ComputeAbsolute: Compute absolute expression using ERCC spike ins

In grandR: Comprehensive Analysis of Nucleotide Conversion Sequencing Data

Compute absolute expression using ERCC spike ins

Description

Compute absolute expression in a grandR object and puts the normalized data into a new slot

Usage

ComputeAbsolute(
  data,
  dilution = 40000,
  volume = 10,
  slot = "tpm",
  name = "absolute"
)

Arguments

data	the grandR object
dilution	the dilution of the spikein transcript in the lysis reaction mix
volume	the approximate volume of the lysis chamber (nanoliters)
slot	the slot containing relative expression values
name	the name of the new slot to put absolute expression values in

Value

a new grandR object with an additional slot

See Also

relative2abs

grandR documentation built on April 4, 2025, 2:27 a.m.