TransformSnapshot: Estimate parameters for a one-shot experiment.

In grandR: Comprehensive Analysis of Nucleotide Conversion Sequencing Data

Estimate parameters for a one-shot experiment.

Description

Under steady state conditions it is straight-forward to estimate s and d. Otherwise, the total levels at some other time point are needed.

Usage

TransformSnapshot(ntr, total, t, t0 = NULL, f0 = NULL, full.return = FALSE)

Arguments

ntr	the new to total RNA ratio (measured)
total	the total level of RNA (measured)
t	the labeling duration
t0	time before measurement at which f0 is total level (only necessary under non-steady-state conditions)
f0	total level at t0 (only necessary under non-steady-state conditions)
full.return	also return the provided parameters

Details

t0 must be given as the total time in between the measurement of f0 and the given ntr and total values!

Value

a named vector for s and d

grandR documentation built on April 4, 2025, 2:27 a.m.