ClustreePlot: Clustree Plot

In plotthis: High-Level Plotting Built Upon 'ggplot2' and Other Plotting Packages

Clustree Plot

Description

Creates a clustree (clustering tree) plot visualising how cluster assignments change across increasing clustering resolutions. The plot helps identify stable clustering solutions and understand the hierarchical relationships among clusters at different resolution thresholds.

The function expects a data frame with columns named by a common prefix followed by numeric resolution values (e.g. "res_0.1", "res_0.3", "res_0.5"). Each column contains cluster labels (factor or character) for every observation at that resolution.

Internally, the function uses clustree::clustree() to compute a ggraph-based tree layout where nodes are clusters and edges represent cells transitioning between clusters at adjacent resolutions. Edge colour and width encode the number of transitioning cells.

Key features:

• Resolution-level node colouring — each resolution receives a distinct colour from the selected palette.

• Edge gradient — edges are coloured by transition count using a separate edge_palette colour gradient.

• Flip support — flip = TRUE places resolutions on the x-axis for left-to-right reading.

• Split by groups — split_by generates per-group clustree plots that are combined via patchwork.

• Automatic dimensions — plot height and width are automatically computed based on the number of resolutions, clusters, and the legend configuration.

Usage

ClustreePlot(
  data,
  prefix,
  flip = FALSE,
  split_by = NULL,
  split_by_sep = "_",
  palette = "Paired",
  palcolor = NULL,
  palreverse = FALSE,
  edge_palette = "Spectral",
  edge_palcolor = NULL,
  aspect.ratio = 1,
  legend.position = "right",
  legend.direction = "vertical",
  title = NULL,
  subtitle = NULL,
  xlab = NULL,
  ylab = NULL,
  expand = c(0.1, 0.1),
  theme = "theme_this",
  theme_args = list(),
  combine = TRUE,
  nrow = NULL,
  ncol = NULL,
  byrow = TRUE,
  seed = 8525,
  axes = NULL,
  axis_titles = axes,
  guides = NULL,
  design = NULL,
  ...
)

Arguments

data	A data frame.
prefix	A character string specifying the common prefix of the resolution columns in data. All columns whose names start with this prefix are selected as resolution columns. The suffix after the prefix is parsed as a numeric resolution value. Supports "_" and "." as separators between the prefix and the resolution value (e.g. "res_0.5" or "p.0.5").
flip	A logical value. If TRUE, the tree is flipped so that resolutions are displayed on the x-axis (left to right) and cluster assignments are shown as row labels on the y-axis. Default: FALSE.
split_by	The column(s) to split data by and generate separate clustree plots for each level. Each split level produces an independent clustree plot via ClustreePlotAtomic.
split_by_sep	A character string used to concatenate multiple split_by column values when split_by specifies more than one column. Default: "_".
palette	A character string specifying the palette to use. A named list or vector can be used to specify the palettes for different split_by values.
palcolor	A character string specifying the color to use in the palette. A named list can be used to specify the colors for different split_by values. If some values are missing, the values from the palette will be used (palcolor will be NULL for those values).
palreverse	A logical value indicating whether to reverse the palette. Default is FALSE.
edge_palette	A character string specifying the palette name for the edge colour gradient. Edges are coloured by the number of transitioning cells between clusters at adjacent resolutions, using ggraph::scale_edge_color_gradientn(). Default: "Spectral".
edge_palcolor	A character vector of custom colours for the edge colour gradient. When NULL (the default), colours are derived from edge_palette.
aspect.ratio	A numeric value specifying the aspect ratio of the plot.
legend.position	A character string specifying the position of the legend. if waiver(), for single groups, the legend will be "none", otherwise "right".
legend.direction	A character string specifying the direction of the legend.
title	A character string specifying the title of the plot. A function can be used to generate the title based on the default title. This is useful when split_by is used and the title needs to be dynamic.
subtitle	A character string specifying the subtitle of the plot.
xlab	A character string specifying the x-axis label.
ylab	A character string specifying the y-axis label.
expand	The values to expand the x and y axes. It is like CSS padding. When a single value is provided, it is used for both axes on both sides. When two values are provided, the first value is used for the top/bottom side and the second value is used for the left/right side. When three values are provided, the first value is used for the top side, the second value is used for the left/right side, and the third value is used for the bottom side. When four values are provided, the values are used for the top, right, bottom, and left sides, respectively. You can also use a named vector to specify the values for each side. When the axis is discrete, the values will be applied as 'add' to the 'expansion' function. When the axis is continuous, the values will be applied as 'mult' to the 'expansion' function. See also https://ggplot2.tidyverse.org/reference/expansion.html
theme	A character string or a theme class (i.e. ggplot2::theme_classic) specifying the theme to use. Default is "theme_this".
theme_args	A list of arguments to pass to the theme function.
combine	A logical value. If TRUE (the default), the list of per-split plots is combined into a single patchwork object. If FALSE, returns the raw list of ggplot objects.
nrow, ncol, byrow	Integers controlling the layout of combined plots via patchwork::wrap_plots(). byrow = TRUE (default) fills the layout row-wise. Ignored when design is provided.
seed	The random seed for reproducibility. Passed to validate_common_args(). Default: 8525.
axes, axis_titles	Strings controlling how axes and axis titles are handled across combined plots. Passed to combine_plots(). See ?patchwork::wrap_plots for options ("keep", "collect", "collect_x", "collect_y").
guides	A string controlling guide collection across combined plots. Passed to combine_plots().
design	A custom layout specification for combined plots. Passed to combine_plots(). When specified, nrow, ncol, and byrow are ignored.
...	Additional arguments passed to clustree::clustree(). Commonly used overrides include node_size_range, node_text_size, layout (default: "sugiyama"), show_axis, and node_text_colour. Note that x (the data) and prefix are set internally and cannot be overridden here.

Value

A ggplot object (single plot), a patchwork object (when combine = TRUE with split_by), or a list of ggplot objects (when combine = FALSE).

split_by Workflow (ClustreePlot)

When split_by is provided, the following pipeline executes:

1. Argument validation — validate_common_args() checks the seed value and sets the random seed.

2. Theme resolution — process_theme() resolves the theme string or function to a theme function.

3. Split column validation — check_columns() resolves split_by with force_factor = TRUE, allow_multi = TRUE, concat_multi = TRUE.

4. Data splitting — splits data by split_by levels (unused levels dropped), preserving factor level order.

5. Per-split palette / colour / legend — check_palette(), check_palcolor(), and check_legend() resolve per-split overrides for palette, palcolor, legend.position, and legend.direction.

6. Per-split title — when title is a function, it receives the default title (the split level name) and can return a custom string; otherwise title %||% split_level is used.

7. Dispatch — each split subset is passed to ClustreePlotAtomic with the per-split parameters.

8. Combination — combine_plots() assembles the list of plots via patchwork::wrap_plots, honouring nrow/ncol/byrow/design.

Examples

set.seed(8525)
N <- 100
data <- data.frame(
    p.0.4 = sample(LETTERS[1:5], N, replace = TRUE),
    p.0.5 = sample(LETTERS[1:6], N, replace = TRUE),
    p.0.6 = sample(LETTERS[1:7], N, replace = TRUE),
    p.0.7 = sample(LETTERS[1:8], N, replace = TRUE),
    p.0.8 = sample(LETTERS[1:9], N, replace = TRUE),
    p.0.9 = sample(LETTERS[1:10], N, replace = TRUE),
    p.1 = sample(LETTERS[1:30], N, replace = TRUE),
    split = sample(1:2, N, replace = TRUE)
)

# --- Basic clustree plot ---
ClustreePlot(data, prefix = "p")

# --- Flipped layout (resolutions on x-axis) ---
ClustreePlot(data, prefix = "p", flip = TRUE)

# --- Split by group ---
ClustreePlot(data, prefix = "p", split_by = "split")

# --- Split by group with per-split palettes ---
ClustreePlot(data, prefix = "p", split_by = "split",
             palette = c("1" = "Set1", "2" = "Paired"))

plotthis documentation built on July 9, 2026, 5:07 p.m.