| DimPlotAtomic | R Documentation |
Core implementation for dimension reduction visualisation. This is the
internal workhorse dispatched by both DimPlot() (group-based) and
FeatureDimPlot() (continuous feature expression). It renders a 2D
scatter plot of ordination axes with extensive annotation capabilities,
and automatically delegates to DimPlotAtomic3D() for interactive 3D
plots when three dims are provided.
The function supports two primary colouring modes:
group_by — discrete factor colouring with a legend of group levels, optional density/statistical overlays, and group mark / label annotations.
features — continuous numeric colouring with a gradient colour bar,
multi-feature faceting via tidyr::pivot_longer(), and optional cutoff
/ quantile trimming via prepare_continuous_color_scale().
Additional annotation layers include: graph / network edges drawn as
segments between connected nodes, 2D density contours (filled or outline),
group marks (hull, ellipse, rect, circle via ggforce), lineage curves
(LOESS-smoothed paths with optional whiskers), velocity / RNA-velocity
arrows (raw, grid, or stream via VelocityPlot()), statistical summary
mini-plots (pie, ring, bar, line) embedded at group centroids, background
points from other facets (faded context), and group labels with repulsion
(via ggrepel::geom_text_repel()).
Rendering scales automatically: scatter points for small datasets,
scattermore::geom_scattermore() raster for n > 1e5, or hex-binned
aggregation (stat_summary_hex() / geom_hex()). Legend assembly uses
cowplot::get_plot_component() with independent guide-boxes for base
groups, lineages, velocity, and stat-by annotations — combined via
rbind / cbind and re-inserted with add_grob().
DimPlotAtomic(
data,
dims = 1:2,
group_by = NULL,
group_by_sep = "_",
features = NULL,
lower_quantile = 0,
upper_quantile = 0.99,
lower_cutoff = NULL,
upper_cutoff = NULL,
pt_size = NULL,
pt_alpha = 1,
bg_color = "grey80",
bg_cutoff = NULL,
color_name = "",
label_insitu = FALSE,
show_stat = !identical(theme, "theme_blank"),
label = FALSE,
label_size = 4,
label_fg = "white",
label_bg = "black",
label_bg_r = 0.1,
label_repel = FALSE,
label_repulsion = 20,
label_pt_size = 1,
label_pt_color = "black",
label_segment_color = "black",
order = c("as-is", "reverse", "high-top", "low-top", "random"),
highlight = NULL,
highlight_alpha = 1,
highlight_size = 1,
highlight_color = "black",
highlight_stroke = 0.8,
add_mark = FALSE,
mark_type = c("hull", "ellipse", "rect", "circle"),
mark_expand = unit(3, "mm"),
mark_alpha = 0.1,
mark_linetype = 1,
stat_by = NULL,
stat_plot_type = c("pie", "ring", "bar", "line"),
stat_plot_size = 0.1,
stat_palette = "Set1",
stat_args = list(),
graph = NULL,
edge_size = c(0.05, 0.5),
edge_alpha = 0.1,
edge_color = "grey40",
add_density = FALSE,
density_color = "grey80",
density_filled = FALSE,
density_filled_palette = "Greys",
density_filled_palcolor = NULL,
lineages = NULL,
lineages_trim = c(0.01, 0.99),
lineages_span = 0.75,
lineages_palette = "Dark2",
lineages_palcolor = NULL,
lineages_arrow = ggplot2::arrow(length = unit(0.1, "inches")),
lineages_linewidth = 1,
lineages_line_bg = "white",
lineages_line_bg_stroke = 0.5,
lineages_whiskers = FALSE,
lineages_whiskers_linewidth = 0.5,
lineages_whiskers_alpha = 0.5,
velocity = NULL,
velocity_plot_type = c("raw", "grid", "stream"),
velocity_n_neighbors = NULL,
velocity_density = 1,
velocity_smooth = 0.5,
velocity_scale = 1,
velocity_min_mass = 1,
velocity_cutoff_perc = 5,
velocity_group_palette = "Set2",
velocity_group_palcolor = NULL,
arrow_angle = 20,
arrow_color = "black",
streamline_l = 5,
streamline_minl = 1,
streamline_res = 1,
streamline_n = 15,
arrow_alpha = 1,
streamline_width = c(0, 0.8),
streamline_alpha = 1,
streamline_color = NULL,
streamline_palette = "RdYlBu",
streamline_palcolor = NULL,
streamline_bg_color = "white",
streamline_bg_stroke = 0.5,
keep_na = FALSE,
keep_empty = FALSE,
facet_by = NULL,
facet_scales = "fixed",
facet_nrow = NULL,
facet_ncol = NULL,
facet_byrow = TRUE,
title = NULL,
subtitle = NULL,
xlab = NULL,
ylab = NULL,
theme = "theme_this",
theme_args = list(),
aspect.ratio = 1,
legend.position = "right",
legend.direction = "vertical",
raster = NULL,
raster_dpi = c(512, 512),
hex = FALSE,
hex_linewidth = 0.5,
hex_count = !is.null(group_by),
hex_bins = 50,
hex_binwidth = NULL,
palette = ifelse(is.null(features), "Paired", "Spectral"),
palcolor = NULL,
palreverse = FALSE,
seed = 8525,
...
)
data |
A data frame. |
dims |
A character vector of the column names to plot on the x, y (and optionally z) axes or a numeric vector of the column indices. When 3 dimensions are provided, a 3D interactive plot is created using plotly. Supported in 3D: group_by, features, labels, highlight, lineages, graph/network, show_stat, order. Not supported in 3D: add_mark, stat_by, add_density, velocity, hex, facet_by, raster. |
group_by |
A character string of the column name to group the data by for discrete colouring.
A character/factor column is expected. If multiple columns are provided, the columns will be concatenated with |
group_by_sep |
A character string to concatenate the columns in |
features |
A character vector of the column names to plot as features (continuous colouring).
When multiple features are provided and |
lower_quantile, upper_quantile |
Lower and upper quantiles for the continuous color/fill scale.
The actual cutoffs are determined by these quantiles when |
lower_cutoff, upper_cutoff |
Explicit lower and upper cutoffs for the continuous color/fill scale.
When |
pt_size |
A numeric value of the point size. If NULL (default), the point size is auto-calculated as
|
pt_alpha |
A numeric value in |
bg_color |
A character string specifying the colour used for NA-valued points and
background context points drawn from other facets. Default is |
bg_cutoff |
A numeric threshold. Feature values with absolute value below this
cutoff are set to |
color_name |
A character string used as the title for the continuous colour bar
in feature mode. Default is |
label_insitu |
A logical value. If |
show_stat |
A logical value. If |
label |
A logical value. If |
label_size |
A numeric value for the label text size. Passed to |
label_fg |
A character string for the label text (foreground) colour. Default is |
label_bg |
A character string for the label background / outline colour. Default is |
label_bg_r |
A numeric value for the background fill ratio of the label bounding box.
Passed to |
label_repel |
A logical value. If |
label_repulsion |
A numeric value for the repulsion force when |
label_pt_size |
A numeric value for the size of the anchor point drawn when
|
label_pt_color |
A character string for the colour of the label anchor point. Default is |
label_segment_color |
A character string for the colour of the line segment connecting
the label to the anchor. Used in non-repel mode ( |
order |
A character string controlling the draw order of points:
For |
highlight |
A specification for highlighted points:
|
highlight_alpha |
A numeric value in |
highlight_size |
A numeric value for the size of the inner (coloured) highlight point.
Default is |
highlight_color |
A character string for the colour of the outer highlight ring.
Default is |
highlight_stroke |
A numeric value for the thickness of the outer highlight ring (the
difference between the outer ring size and |
add_mark |
A logical value. If |
mark_type |
A character string specifying the mark shape. Options:
|
mark_expand |
A unit value for the outward expansion of the mark boundary.
Passed to |
mark_alpha |
A numeric value in |
mark_linetype |
A numeric value for the line type of the mark boundary. Default is |
stat_by |
A character string naming a column used to compute per-group statistical summary
mini-plots embedded at group centroid positions. Only supported with |
stat_plot_type |
A character string specifying the mini-plot type. Options:
|
stat_plot_size |
A numeric value for the size of the stat mini-plot, expressed as a
fraction of the axis range. Default is |
stat_args |
A list of additional arguments passed to the stat plot function
(e.g., |
graph |
A specification for network / graph edges to overlay. Sources:
|
edge_size |
A numeric vector of length 2 specifying the range |
edge_alpha |
A numeric value in |
edge_color |
A character string for the colour of graph edges. Default is |
add_density |
A logical value. If |
density_color |
A character string for the colour of the density contour lines.
Used when |
density_filled |
A logical value. If |
density_filled_palette |
A character string naming the palette for the filled density layer.
Default is |
density_filled_palcolor |
A character vector of specific colours for the filled density
palette. Default is |
lineages |
A character vector of column names representing pseudotime / trajectory lineages.
Each column is fitted with a LOESS smooth ( |
lineages_trim |
A numeric vector of length 2 specifying the lower and upper quantile
thresholds |
lineages_span |
A numeric value passed as |
lineages_palette |
A character string naming the palette for lineage colours.
Default is |
lineages_palcolor |
A character vector of specific colours for lineage curves.
Default is |
lineages_arrow |
A ggplot2 |
lineages_linewidth |
A numeric value for the width of the lineage curve lines.
Default is |
lineages_line_bg |
A character string for the colour of the background (wider) stroke
drawn behind each lineage curve for improved visibility. Default is |
lineages_line_bg_stroke |
A numeric value for the additional width of the background
stroke relative to |
lineages_whiskers |
A logical value. If |
lineages_whiskers_linewidth |
A numeric value for the width of the whisker lines.
Default is |
lineages_whiskers_alpha |
A numeric value in |
velocity |
A specification for RNA-velocity arrows. Can be:
|
velocity_plot_type |
A character string specifying the velocity rendering style.
Options: |
velocity_n_neighbors |
A numeric value for the number of neighbours used in the
velocity grid computation. Default is |
velocity_density |
A numeric value for the velocity kernel density bandwidth.
Default is |
velocity_smooth |
A numeric value for the velocity smoothing parameter.
Default is |
velocity_scale |
A numeric value for scaling the velocity arrows. Default is |
velocity_min_mass |
A numeric value for the minimum cell mass threshold in velocity
grid computation. Default is |
velocity_cutoff_perc |
A numeric value for the velocity cutoff percentage.
Default is |
velocity_group_palette |
A character string naming the palette for velocity group
colours (used in |
velocity_group_palcolor |
A character vector of specific colours for velocity groups.
Default is |
arrow_angle |
A numeric value specifying the angle of the arrowheads in degrees. Applied to |
arrow_color |
A character string specifying the color of the velocity arrows. For |
streamline_l |
A numeric value specifying the integration length of the streamlines. Passed to |
streamline_minl |
A numeric value specifying the minimum streamline length. Shorter streamlines are not drawn. Passed to |
streamline_res |
A numeric value specifying the resolution of the streamline integration. Passed to |
streamline_n |
A numeric value specifying the number of streamlines to draw. Passed to |
arrow_alpha |
A numeric value between 0 and 1 specifying the transparency of the velocity arrows. Only used when |
streamline_width |
A numeric vector of length 2 specifying the range of line widths for streamlines. Passed to |
streamline_alpha |
A numeric value between 0 and 1 specifying the transparency of the velocity streamlines. Default is 1. |
streamline_color |
An optional character string specifying a fixed color for streamlines. When |
streamline_palette |
A character string specifying the color palette for streamline velocity magnitude. Passed to |
streamline_palcolor |
An optional character vector of specific colors for the streamline velocity gradient. If |
streamline_bg_color |
A character string specifying the background (outline) color applied to streamlines to create a stroke effect. Default is |
streamline_bg_stroke |
A numeric value specifying the additional line width of the background stroke relative to the foreground streamline. Default is 0.5. |
keep_na |
A logical value or a character to replace the NA values in the data.
It can also take a named list to specify different behavior for different columns.
If TRUE or NA, NA values will be replaced with NA.
If FALSE, NA values will be removed from the data before plotting.
If a character string is provided, NA values will be replaced with the provided string.
If a named vector/list is provided, the names should be the column names to apply the behavior to,
and the values should be one of TRUE, FALSE, or a character string.
Without a named vector/list, the behavior applies to categorical/character columns used on the plot,
for example, the |
keep_empty |
One of FALSE, TRUE and "level". It can also take a named list to specify
different behavior for different columns. Without a named list, the behavior applies to the
categorical/character columns used on the plot, for example, the
|
facet_by |
A character string specifying the column name of the data frame to facet the plot.
Otherwise, the data will be split by |
facet_scales |
Whether to scale the axes of facets. Default is "fixed"
Other options are "free", "free_x", "free_y". See |
facet_nrow |
A numeric value specifying the number of rows in the facet. When facet_by is a single column and facet_wrap is used. |
facet_ncol |
A numeric value specifying the number of columns in the facet. When facet_by is a single column and facet_wrap is used. |
facet_byrow |
A logical value indicating whether to fill the plots by row. Default is TRUE. |
title |
A character string specifying the title of the plot. A function can be used to generate the title based on the default title. This is useful when split_by is used and the title needs to be dynamic. |
subtitle |
A character string specifying the subtitle of the plot. |
xlab |
A character string specifying the x-axis label. |
ylab |
A character string specifying the y-axis label. |
theme |
A character string or a theme class (i.e. ggplot2::theme_classic) specifying the theme to use. Default is "theme_this". |
theme_args |
A list of arguments to pass to the theme function. |
aspect.ratio |
A numeric value specifying the aspect ratio of the plot. |
legend.position |
A character string specifying the position of the legend.
if |
legend.direction |
A character string specifying the direction of the legend. |
raster |
A logical value. If |
raster_dpi |
A numeric vector of length 2 |
hex |
A logical value. If |
hex_linewidth |
A numeric value for the width of the hexagon boundary lines.
Default is |
hex_count |
A logical value. If |
hex_bins |
A numeric value for the number of hex bins along each axis.
Passed to |
hex_binwidth |
A numeric value for the width of individual hex bins.
Passed to |
palette |
A character string specifying the palette to use.
A named list or vector can be used to specify the palettes for different |
palcolor |
A character string specifying the color to use in the palette.
A named list can be used to specify the colors for different |
palreverse |
A logical value indicating whether to reverse the palette. Default is FALSE. |
seed |
The random seed to use. Default is 8525. |
... |
Additional arguments. |
A ggplot object or a plotly object (when 3 dimensions are provided)
DimPlotAtomic executes the following steps:
ggplot dispatch — selects gglogger::ggplot or ggplot2::ggplot.
Order validation — match.arg(order).
Dimension resolution — converts numeric indices to column names,
validates exactly 2 or 3 dims via check_columns().
Column resolution — validates group_by (force_factor, concat_multi),
features, and facet_by. Requires at least one of group_by or
features. Blocks facet_by when multiple features are present (features
themselves become facets).
Auto defaults — pt_size defaults to min(3000 / nrow(data), 0.6);
raster auto-enables when nrow(data) > 1e5; theme_blank suppresses
axis labels.
Label force-enable — if label_repel or label_insitu is TRUE,
label is forced to TRUE with a message.
NA / empty handling — process_keep_na_empty() filters the data;
keep_empty values extracted for group_by and facet_by.
group_by preprocessing — group values / colours resolved via
palette_this(). NA levels mapped to literal "NA" string.
label_use is constructed based on label, label_insitu, and
show_stat combinations. Facet labeller is set to as_labeller() with
per-facet N annotations when show_stat = TRUE.
Multi-feature pivot — when length(features) > 1, data is pivoted
to long format via tidyr::pivot_longer() with .feature as the facet
variable and .value as the numeric column.
Continuous colour scale — when features is set,
prepare_continuous_color_scale() applies quantile/cutoff trimming
and returns anchor values for the gradient.
Point ordering — applies the selected order strategy (as-is,
reverse, high-top, low-top, random) by sorting or shuffling rows.
3D branch — if length(dims) == 3, emits a warning for unsupported
features, optionally down-samples with stratified group sampling (when
raster = TRUE), and delegates to DimPlotAtomic3D().
Group marks (2D only, when add_mark = TRUE) — dispatches to
ggforce::geom_mark_hull/ellipse/rect/circle, with new_scale_fill()
/ new_scale_color() to isolate mark scales.
Graph / network (2D only) — resolves the graph source (same logic
as 3D), melts the matrix, handles faceted data by per-facet edge
splitting, and adds geom_segment(aes(linewidth = value)) with
scale_linewidth_continuous().
Density overlay (2D only) — filled (stat_density_2d(geom = "raster"))
or outline (geom_density_2d()).
Base scales and theme — x / y limits from data range,
do_call(theme, theme_args), aspect ratio and legend position.
Background points for facet context — for faceted plots (excluding multi-feature auto-faceting), points from other facets are added as faded background (raster / hex / point depending on settings).
Main point layer — dispatches by rendering mode:
raster: scattermore::geom_scattermore() with separate NA / non-NA
group layers.
hex: geom_hex() (group_by with optional count alpha, or
stat_summary_hex() for features). Raises has_fill = TRUE.
standard: geom_point().
Highlight (2D only) — resolves highlight specification (TRUE, filter expression, row names, indices), errors on hex + highlight combo, renders highlight points with stroke (outer ring + inner colour).
Colour scales — scale_color_manual() for group_by (with
keep_empty-aware breaks/limits/drop), or
scale_color_gradientn() for features. scale_fill_manual() /
scale_fill_gradientn() added when has_fill is TRUE.
Base legend — extracted via cowplot::get_plot_component("guide-box-bottom").
Lineages (2D only, no facet_by) — per-lineage LOESS fitting
(span = lineages_span, degree = 2) with optional whiskers connecting
smoothed to raw coordinates. Rendered as geom_path() with background
stroke + foreground colour, plus scale_color_manual(). Legend extracted
as a separate guide-box.
Velocity (2D only, no facet_by) — delegates to VelocityPlot() with
return_layer = TRUE. Adds new_scale_color() when the velocity layer
has its own colour scale. Legend extracted separately.
Stat-by mini-plots (2D only, no facet_by, no features) — dispatches
to PieChart() / RingPlot() / BarPlot() / LinePlot() via
do_call(). Each mini-plot is rendered as annotation_custom() at the
group's median coordinates, scaled by stat_plot_size * range.
Legend extracted from the stat plot.
Group labels (2D only, no features) — geom_text_repel() with
optional repulsion (force = label_repulsion) or fixed segments
(force = 0, bold, min.segment.length = 0). Labels positioned at
group median coordinates (computed per facet if applicable).
Legend assembly — when additional legends exist (lineages, velocity,
stat_by), they are combined with the base legend via cbind (vertical
direction) or rbind (horizontal). The combined legend is re-inserted
via add_grob(gtable, legend, legend.position).
Dimension calculation — calculate_plot_dimensions(base_height = 5.5, aspect.ratio, legend_n, legend_nchar) sets height / width attributes.
Faceting — facet_plot() is called only when no additional legends
were assembled (otherwise the combined-legend grob is returned directly).
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