inferGenotype: Infer a subject-specific genotype using a frequency method

In tigger: Infers Novel Immunoglobulin Alleles from Sequencing Data

Infer a subject-specific genotype using a frequency method

Description

inferGenotype infers an subject's genotype using a frequency method. The genotype is inferred by finding the minimum number set of alleles that can explain the majority of each gene's calls. The most common allele of each gene is included in the genotype first, and the next most common allele is added until the desired fraction of alleles can be explained. In this way, mistaken allele calls (resulting from sequences which by chance have been mutated to look like another allele) can be removed.

Usage

inferGenotype(
  data,
  germline_db = NA,
  novel = NA,
  v_call = "v_call",
  seq = "sequence_alignment",
  fraction_to_explain = 0.875,
  gene_cutoff = 1e-04,
  find_unmutated = TRUE
)

Arguments

data	data.frame containing V allele calls from a single subject.
germline_db	named vector of sequences containing the germline sequences named in allele_calls. Only required if find_unmutated is TRUE.
novel	optional data.frame of the type novel returned by findNovelAlleles containing germline sequences that will be utilized if find_unmutated is TRUE. See Details.
v_call	column in data with V allele calls. Default is "v_call".
seq	name of the column in data with the aligned, IMGT-numbered, V(D)J nucleotide sequence. Default is sequence_alignment.
fraction_to_explain	the portion of each gene that must be explained by the alleles that will be included in the genotype.
gene_cutoff	either a number of sequences or a fraction of the length of allele_calls denoting the minimum number of times a gene must be observed in allele_calls to be included in the genotype.
find_unmutated	if TRUE, use germline_db to find which samples are unmutated. Not needed if allele_calls only represent unmutated samples.

Details

Allele calls representing cases where multiple alleles have been assigned to a single sample sequence are rare among unmutated sequences but may result if nucleotides for certain positions are not available. Calls containing multiple alleles are treated as belonging to all groups. If novel is provided, all sequences that are assigned to the same starting allele as any novel germline allele will have the novel germline allele appended to their assignent prior to searching for unmutated sequences.

Value

A data.frame of alleles denoting the genotype of the subject containing the following columns:

• gene: The gene name without allele.

• alleles: Comma separated list of alleles for the given gene.

• counts: Comma separated list of observed sequences for each corresponding allele in the alleles list.

• total: The total count of observed sequences for the given gene.

• note: Any comments on the inferrence.

Note

This method works best with data derived from blood, where a large portion of sequences are expected to be unmutated. Ideally, there should be hundreds of allele calls per gene in the input.

See Also

plotGenotype for a colorful visualization and genotypeFasta to convert the genotype to nucleotide sequences. See inferGenotypeBayesian to infer a subject-specific genotype using a Bayesian approach.

Examples

# Infer IGHV genotype, using only unmutated sequences, including novel alleles
inferGenotype(AIRRDb, germline_db=SampleGermlineIGHV, novel=SampleNovel,
              find_unmutated=TRUE)

tigger documentation built on Oct. 10, 2023, 5:08 p.m.