manhattan | R Documentation |
manhattan()
displays association results for the entire genome on a Manhattan plot.
Required parameter is at least one dataset (dataframe) containing the association data (with columns CHROM,POS,P
in upper or lowercase)
All other input parameters are optional
manhattan(
df,
ntop = 4,
title = "",
annotate = NULL,
color = NULL,
sign_thresh = 5e-08,
sign_thresh_color = "red",
sign_thresh_label_size = 3.5,
label_size = 3.5,
size = 0.8,
shape = 19,
alpha = 1,
highlight_genes_color = "darkred",
highlight_genes_ypos = 1.5,
axis_text_size = 12,
axis_title_size = 14,
title_text_size = 15,
legend_title_size = 13,
legend_text_size = 12,
protein_coding_only = TRUE,
angle = 0,
legend_labels = NULL,
chr = NULL,
annotate_with = "Gene_Symbol",
region_size = 2e+07,
legend_name = NULL,
legend_position = "bottom",
nudge_x = 0.1,
nudge_y = 0.7,
xmin = NULL,
xmax = NULL,
ymin = NULL,
ymax = NULL,
highlight_genes = NULL,
label_color = NULL,
legend_nrow = NULL,
gene_label_size = NULL,
gene_label_angle = 0,
scale = 1,
show_legend = TRUE,
sign_thresh_linetype = "dashed",
sign_thresh_size = 0.5,
rsids = NULL,
rsids_color = NULL,
rsids_with_vline = NULL,
annotate_with_vline = NULL,
shades_color = NULL,
shades_alpha = 0.5,
segment.size = 0.2,
segment.color = "black",
segment.linetype = "dashed",
max.overlaps = 10,
label_fontface = "plain",
label_family = "",
gene_label_fontface = "plain",
gene_label_family = "",
build = 38,
verbose = NULL,
label_alpha = 1,
shades_line_alpha = 1,
vline = NULL,
vline_color = "grey",
vline_linetype = "dashed",
vline_alpha = 1,
vline_size = 0.5,
region = NULL,
theme_grey = FALSE,
xaxis_label = "Chromosome",
use_shades = FALSE,
even_no_chr_lightness = 0.8,
get_chr_lengths_from_data = TRUE,
log_trans_p = TRUE,
chr_ticknames = NULL,
show_all_chrticks = FALSE,
hide_chrticks_from_pos = 17,
hide_chrticks_to_pos = NULL,
hide_every_nth_chrtick = 2,
downsample_cutoff = 0.05,
downsample_prop = 0.1
)
df |
Dataframe or a list of dataframes (required columns are |
ntop |
An integer, number of datasets (GWAS results) to show on the top plot |
title |
A string to set the plot title |
annotate |
A number (p-value). Display annotation for variants with p-values below this threshold |
color |
A string or a vector of strings, for setting the color of the datapoints on the plot |
sign_thresh |
A number or vector of numbers, setting the horizontal significance threshold (default: |
sign_thresh_color |
A string or vector of strings to set the color/s of the significance threshold/s |
sign_thresh_label_size |
A number setting the text size of the label for the significance thresholds (default text size is 3.5) |
label_size |
An number to set the size of the plot labels (default: |
size |
A number or a vector of numbers, setting the size of the plot points (default: |
shape |
A number of a vector of numbers setting the shape of the plotted points |
alpha |
A number or a vector of numbers setting the transparency of the plotted points |
highlight_genes_color |
A string, color for the highlighted genes (default: darkred) |
highlight_genes_ypos |
An integer, controlling where on the y-axis the highlighted genes are placed (default value is 1) |
axis_text_size |
A number, size of the x and y axes tick labels (default: 12) |
axis_title_size |
A number, size of the x and y title labels (default: 12) |
title_text_size |
A number, size of the plot title (default: 13) |
legend_title_size |
A number, size of the legend title |
legend_text_size |
A number, size of the legend text |
protein_coding_only |
A logical scalar, if TRUE, only protein coding genes are used for annotation |
angle |
A number, the angle of the text label |
legend_labels |
A string or vector of strings representing legend labels for the input datasets |
chr |
A string or integer, the chromosome to plot (i.e. chr15), only required if the input dataframe contains results from more than one chromosome |
annotate_with |
A string. Annotate the variants with either Gene_Symbol or ID (default: "Gene_Symbol") |
region_size |
An integer (default = 20000000) (or a string represented as 200kb or 2MB) indicating the window size for variant labeling. Increase this number for sparser annotation and decrease for denser annotation. |
legend_name |
A string, use to change the name of the legend (default: None) |
legend_position |
A string, top,bottom,left or right |
nudge_x |
A number to vertically adjust the starting position of each gene label (this is a ggrepel parameter) |
nudge_y |
A number to horizontally adjust the starting position of each gene label (this is a ggrepel parameter) |
xmin , xmax |
Integer, setting the chromosomal range to display on the x-axis |
ymin , ymax |
Integer, min and max of the y-axis, (default values: |
highlight_genes |
A string or vector of strings, gene or genes to highlight at the bottom of the plot |
label_color |
A string or a vector of strings. To change the color of the gene or variant labels |
legend_nrow |
An integer, sets the number of rows allowed for the legend labels |
gene_label_size |
A number setting the size of the gene labels shown at the bottom of the plot |
gene_label_angle |
A number setting the angle of the gene label shown at the bottom of the plot (default: 0) |
scale |
A number, to change the size of the title and axes labels and ticks at the same time (default : 1) |
show_legend |
A logical scalar, set to FALSE to hide the legend (default : TRUE) |
sign_thresh_linetype |
A string, the line-type of the horizontal significance threshold (default : dashed) |
sign_thresh_size |
A number, sets the size of the horizontal significance threshold line (default : 1) |
rsids |
A string (rsid) or vector of strings to highlight on the plot, e.g. |
rsids_color |
A string, the color of the variants in variants_id (default color is red) |
rsids_with_vline |
A string (rsid) or vector of strings to highlight on the plot with their rsids and vertical lines further highlighting their positions |
annotate_with_vline |
A number (p-value). Display annotation and vertical lines for variants with p-values below this threshold |
shades_color |
The color of the rectangles (shades) representing the different chromosomes on the Manhattan plot |
shades_alpha |
The transparency (alpha) of the rectangles (shades) |
segment.size |
line segment color (ggrepel argument) |
segment.color |
line segment thickness (ggrepel argument) |
segment.linetype |
line segment solid, dashed, etc.(ggrepel argument) |
max.overlaps |
Exclude text labels that overlap too many things. Defaults to 10 (ggrepel argument) |
label_fontface |
A string or a vector of strings. Label font “plain”, “bold”, “italic”, “bold.italic” (ggrepel argument) |
label_family |
A string or a vector of strings. Label font name (default ggrepel argument is "") |
gene_label_fontface |
Gene label font “plain”, “bold”, “italic”, “bold.italic” (ggrepel argument) |
gene_label_family |
Gene label font name (default ggrepel argument is "") |
build |
A number representing the genome build or a data frame. Set to 37 to change to build (GRCh37). The default is build 38 (GRCh38). |
verbose |
A logical scalar (default: NULL). Set to FALSE to suppress printed messages |
label_alpha |
An number or vector of numbers to set the transparency of the plot labels (default: |
shades_line_alpha |
The transparency (alpha) of the lines around the rectangles (shades) |
vline |
A number or vector of numbers to add a vertical line to the plot at a specific chromosomal position, e.g |
vline_color |
A string. The color of added vertical line/s (default: grey) |
vline_linetype |
A string. The linetype of added vertical line/s (default : dashed) |
vline_alpha |
A number. The alpha of added vertical line/s (default : 1) |
vline_size |
A number.The size of added vertical line/s (default : 0.5) |
region |
A string representing a genetic region, e.g. chr1:67038906-67359979 |
theme_grey |
A logical scalar (default: FALSE). Use gray rectangles (instead of white to distinguish between chromosomes) |
xaxis_label |
A string. The label for the x-axis (default: Chromosome) |
use_shades |
A logical scalar (default: FALSE). Use shades/rectangles to distinguish between chromosomes |
even_no_chr_lightness |
Lightness value for even numbered chromosomes. A number or vector of numbers between 0 and 1 (default: 0.8). If set to 0.5, the same color as shown for odd numbered chromosomes is displayed. A value below 0.5 will result in a darker color displayed for even numbered chromosomes, whereas a value above 0.5 results in a lighter color. |
get_chr_lengths_from_data |
A logical scalar (default: TRUE). If set to FALSE, use the inbuilt chromosome lengths (from hg38), instead of chromosome lengths based on the max position for each chromosome in the input dataset/s. |
log_trans_p |
A logical scalar (default: TRUE). By default the p-values in the input datasets are log transformed using -log10. Set this argument to FALSE if the p-values in the datasets have already been log transformed. |
chr_ticknames |
A vector containing the chromosome names displayed on the x-axis. If NULL, the following format is used: chr_ticknames <- c(1:16, ”,18, ”,20, ”,22, 'X') |
show_all_chrticks |
A logical scalar (default : FALSE). Set to TRUE to show all the chromosome names on the ticks on the x-axis |
hide_chrticks_from_pos |
A number (default: 17). Hide every nth chromosome name on the x-axis FROM this position (chromosome number) |
hide_chrticks_to_pos |
A number (default: NULL). Hide every nth chromosome name on the x-axis TO this position (chromosome number). When NULL this variable will be set to the number of numeric chromosomes in the input dataset. |
hide_every_nth_chrtick |
A number (default: 2). Hide every nth chromosome tick on the x-axis (from the hide_chr_ticks_from_pos to the hide_chr_ticks_to_pos). |
downsample_cutoff |
A number (default: 0.05) used to downsample the input dataset prior to plotting. Sets the fraction of high p-value (default: P>0.05) markers to display on the plot. |
downsample_prop |
A number (default: 0.1) used to downsample the input dataset prior to plotting. Only a proportion of the variants (10% by default) with P-values higher than the downsample_cutoff will be displayed on the plot. |
ggplot object
## Not run:
manhattan(CD_UKBB)
## End(Not run)
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.