anova.cca: Permutation Test for Constrained Correspondence Analysis,...

anova.ccaR Documentation

Permutation Test for Constrained Correspondence Analysis, Redundancy Analysis and Constrained Analysis of Principal Coordinates

Description

The function performs an ANOVA like permutation test for Constrained Correspondence Analysis (cca), Redundancy Analysis (rda) or distance-based Redundancy Analysis (dbRDA, capscale) to assess the significance of constraints.

Usage

## S3 method for class 'cca'
anova(object, ..., permutations = how(nperm=999),
     by = NULL, model = c("reduced", "direct", "full"),
     parallel = getOption("mc.cores"), strata = NULL,
     cutoff = 1, scope = NULL)
## S3 method for class 'cca'
permutest(x, permutations = how(nperm = 99),
     model = c("reduced", "direct", "full"), by = NULL, first = FALSE,
     strata = NULL, parallel = getOption("mc.cores"), ...)

Arguments

object

One or several result objects from cca, rda, dbrda or capscale. If there are several result objects, they are compared against each other in the order they were supplied. For a single object, a test specified in by or an overall test is given.

x

A single ordination result object.

permutations

a list of control values for the permutations as returned by the function how, or the number of permutations required, or a permutation matrix where each row gives the permuted indices.

by

Setting by = "axis" will assess significance for each constrained axis, and setting by = "terms" will assess significance for each term (sequentially from first to last), and setting by = "margin" will assess the marginal effects of the terms (each marginal term analysed in a model with all other variables). Function permutest accepts choices "terms" for sequential test of terms, and "onedf" for sequential test of one-degree-of-freedom contrasts.

model

Permutation model: model="direct" permutes community data, model="reduced" permutes residuals of the community data after Conditions (partial model), model = "full" permutes residuals after Conditions and Constraints.

parallel

Use parallel processing with the given number of cores.

strata

An integer vector or factor specifying the strata for permutation. If supplied, observations are permuted only within the specified strata. It is an error to use this when permutations is a matrix, or a how defines blocks. This is a legacy argument that will be deprecated in the future: use permutations = how(..., blocks) instead.

cutoff

Only effective with by="axis" where stops permutations after an axis exceeds the cutoff p-value.

scope

Only effective with by="margin" where it can be used to select the marginal terms for testing. The default is to test all marginal terms in drop.scope.

first

Analyse only significance of the first axis.

...

Parameters passed to other functions. anova.cca passes all arguments to permutest.cca. In anova with by = "axis" you can use argument cutoff (defaults 1) which stops permutations after exceeding the given level.

Details

Functions anova.cca and permutest.cca implement ANOVA like permutation tests for the joint effect of constraints in cca, rda, dbrda or capscale. Function anova is intended as a more user-friendly alternative to permutest (that is the real workhorse).

Function anova can analyse a sequence of constrained ordination models. The analysis is based on the differences in residual deviance in permutations of nested models.

The default test is for the sum of all constrained eigenvalues. Setting first = TRUE will perform a test for the first constrained eigenvalue. Argument first can be set either in anova.cca or in permutest.cca. It is also possible to perform significance tests for each axis or for each term (constraining variable) using argument by in anova.cca. Setting by = "axis" will perform separate significance tests for each constrained axis. All previous constrained axes will be used as conditions (“partialled out”) and a test for the first constrained eigenvalues is performed (Legendre et al. 2011). You can stop permutation tests after exceeding a given significance level with argument cutoff to speed up calculations in large models. Setting by = "terms" will perform separate significance test for each term (constraining variable). The terms are assessed sequentially from first to last, and the order of the terms will influence their significances. Setting by = "margin" will perform separate significance test for each marginal term in a model with all other terms. The marginal test also accepts a scope argument for the drop.scope which can be a character vector of term labels that are analysed, or a fitted model of lower scope. The marginal effects are also known as “Type III” effects, but the current function only evaluates marginal terms. It will, for instance, ignore main effects that are included in interaction terms. In calculating pseudo-F, all terms are compared to the same residual of the full model.

Community data are permuted with choice model="direct", and residuals after partial CCA/ RDA/ dbRDA with choice model="reduced" (default). If there is no partial CCA/ RDA/ dbRDA stage, model="reduced" simply permutes the data and is equivalent to model="direct". The test statistic is “pseudo-F”, which is the ratio of constrained and unconstrained total Inertia (Chi-squares, variances or something similar), each divided by their respective degrees of freedom. If there are no conditions (“partial” terms), the sum of all eigenvalues remains constant, so that pseudo-F and eigenvalues would give equal results. In partial CCA/ RDA/ dbRDA, the effect of conditioning variables (“covariables”) is removed before permutation, and the total Chi-square is not fixed, and test based on pseudo-F would differ from the test based on plain eigenvalues.

Value

The function anova.cca calls permutest.cca and fills an anova table. Additional attributes are Random.seed (the random seeds used), control (the permutation design, see how) and F.perm (the permuted test statistics).

Author(s)

Jari Oksanen

References

Legendre, P. and Legendre, L. (2012). Numerical Ecology. 3rd English ed. Elsevier.

Legendre, P., Oksanen, J. and ter Braak, C.J.F. (2011). Testing the significance of canonical axes in redundancy analysis. Methods in Ecology and Evolution 2, 269–277.

See Also

anova.cca, cca, rda, capscale to get something to analyse. Function drop1.cca calls anova.cca with by = "margin", and add1.cca an analysis for single terms additions, which can be used in automatic or semiautomatic model building (see deviance.cca).

Examples

data(varespec, varechem)
mod <- cca(varespec ~ Al + P + K, varechem)
## overall test
anova(mod)
## tests for individual terms
anova(mod, by="term")
anova(mod, by="margin")
## test for adding all environmental variables
anova(mod, cca(varespec ~ ., varechem))

vegan documentation built on April 18, 2022, 1:05 a.m.