assess_clustering_stability: Assessment of Stability for Graph Clustering

In Core-Bioinformatics/ClustAssess: Tools for Assessing Clustering

Assessment of Stability for Graph Clustering

Description

Evaluates the stability of different graph clustering methods in the clustering pipeline. The method will iterate through different values of the resolution parameter and compare, using the EC Consistency score, the partitions obtained at different seeds.

Usage

assess_clustering_stability(
  graph_adjacency_matrix,
  resolution,
  n_repetitions = 100,
  seed_sequence = NULL,
  ecs_thresh = 1,
  clustering_algorithm = 1:3,
  clustering_arguments = list(),
  verbose = TRUE
)

Arguments

graph_adjacency_matrix	A square adjacency matrix based on which an igraph object will be built. The matrix should have rownames and colnames that correspond to the names of the cells.
resolution	A sequence of resolution values. The resolution parameter controls the coarseness of the clustering. The higher the resolution, the more clusters will be obtained. The resolution parameter is used in the community detection algorithms.
n_repetitions	The number of repetitions of applying the pipeline with different seeds; ignored if seed_sequence is provided by the user. Defaults to 100.
seed_sequence	A custom seed sequence; if the value is NULL, the sequence will be built starting from 1 with a step of 100.
ecs_thresh	The ECS threshold used for merging similar clusterings.
clustering_algorithm	An index or a list of indexes indicating which community detection algorithm will be used: Louvain (1), Louvain refined (2), SLM (3) or Leiden (4). More details can be found in the Seurat's FindClusters function. Defaults to 1:3.
clustering_arguments	A list of additional arguments that will be passed to the clustering method. More details can be found in the Seurat's FindClusters function.
verbose	Boolean value used for displaying the progress bar.

Value

A list having two fields:

• all - a list that contains, for each clustering method and each resolution value, the EC consistency between the partitions obtained by changing the seed

• filtered - similar to all, but for each configuration, we determine the number of clusters that appears the most and use only the partitions with this size

Examples

set.seed(2024)
# create an artificial PCA embedding
pca_embedding <- matrix(runif(100 * 30), nrow = 100)
rownames(pca_embedding) <- paste0("cell_", seq_len(nrow(pca_embedding)))
colnames(pca_embedding) <- paste0("PC_", 1:30)


adj_matrix <- getNNmatrix(
    RANN::nn2(pca_embedding, k = 10)$nn.idx,
    10,
    0,
    -1
)$nn
rownames(adj_matrix) <- paste0("cell_", seq_len(nrow(adj_matrix)))
colnames(adj_matrix) <- paste0("cell_", seq_len(ncol(adj_matrix)))

# alternatively, the adj_matrix can be calculated
# using the `Seurat::FindNeighbors` function.

clust_diff_obj <- assess_clustering_stability(
    graph_adjacency_matrix = adj_matrix,
    resolution = c(0.5, 1),
    n_repetitions = 10,
    clustering_algorithm = 1:2,
    verbose = TRUE
)
plot_clustering_overall_stability(clust_diff_obj)

Core-Bioinformatics/ClustAssess documentation built on Dec. 19, 2024, 8:19 p.m.