Description Usage Arguments Examples
This function takes as input a table containing the gRNA sequences and will output a .fasta and a .gtf file \ containing the merged plasmid + gRNA sequences and the gtf description to add them to a reference genome.
1 2 | create_sgRNA_reference(sgRNA_table, name = "name",
sequence = "sgRNA_sequence", assay = "original", outdir = ".")
|
sgRNA_table |
The table containing the sgRNA sequences and annotation. |
name |
The name of the column in sgRNA_table that should be used to label the fasta sequences |
sequence |
The actual sgRNA sequence |
assay |
The type of CRISPR assay that is being performed. If set to "activation", the tracr backbone is replaced by a tracr MS2 backbone used in VP64 screens. |
outdir |
The directory where the .fasta and .gtf files will be saved. Default = current working directory (".") |
1 |
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