create_sgRNA_reference: A function to create a reference fasta and gtf for plasmid +...

Description Usage Arguments Examples

Description

This function takes as input a table containing the gRNA sequences and will output a .fasta and a .gtf file \ containing the merged plasmid + gRNA sequences and the gtf description to add them to a reference genome.

Usage

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create_sgRNA_reference(sgRNA_table, name = "name",
  sequence = "sgRNA_sequence", assay = "original", outdir = ".")

Arguments

sgRNA_table

The table containing the sgRNA sequences and annotation.

name

The name of the column in sgRNA_table that should be used to label the fasta sequences

sequence

The actual sgRNA sequence

assay

The type of CRISPR assay that is being performed. If set to "activation", the tracr backbone is replaced by a tracr MS2 backbone used in VP64 screens.

outdir

The directory where the .fasta and .gtf files will be saved. Default = current working directory (".")

Examples

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FloWuenne/cropseq.analysis documentation built on May 27, 2019, 3:29 p.m.