R/create_mif.R

In FridleyLab/spatialTIME: Spatial Analysis of Vectra Immunoflourescent Data

#' Create Multiplex Immunoflourescent object 
#'
#' @description Creates an MIF object for use in spatialIF functions
#' @param clinical_data A data frame containing patient level data with one row
#' per participant. 
#' @param sample_data A data frame containing sample level data with one row per 
#' sample. Should at a minimum contain a 2 columns: one for sample names and 
#' one for the corresponding patient name.
#' @param spatial_list A named list of data frames with the spatial data from 
#'  each sample making up each individual data frame
#' @param patient_id A character string indicating the column name for patient id in 
#'  sample and clinical data frames. 
#' @param sample_id A character string indicating the column name for sample id
#'  in the sample data frame
#' 
#' @return Returns a custom MIF
#'    \item{clinical}{Data frame of clinical data}
#'    \item{sample}{Data frame of sample data}
#'    \item{spatial}{Named list of spatial data}
#'    \item{derived}{List of data derived using the MIF object}
#'    \item{patient_id}{The column name for sample id
#'  in the sample data frame with the clinical data}
#'    \item{sample_id}{The column name for sample id
#'  in the sample data frame to merge with the spatial data}
#'    
#' @export
#' @examples
#' #Create mif object
#' library(dplyr)
#' x <- create_mif(clinical_data = example_clinical %>% 
#' mutate(deidentified_id = as.character(deidentified_id)),
#' sample_data = example_summary %>% 
#' mutate(deidentified_id = as.character(deidentified_id)),
#' spatial_list = example_spatial,
#' patient_id = "deidentified_id", 
#' sample_id = "deidentified_sample")

create_mif <- function(clinical_data, sample_data, spatial_list = NULL,
                       patient_id = "patient_id", sample_id = "image_tag"){
  
  sample_data_clean <- sample_data %>% 
    dplyr::full_join(clinical_data %>% 
                dplyr::select(!!patient_id), by = patient_id) %>% 
    dplyr::select(dplyr::all_of(c(!!patient_id, !!sample_id)), dplyr::everything()) %>% 
    dplyr::group_by_at(patient_id) %>% 
    dplyr::mutate(sample_string = paste0(!!(as.name(sample_id)), collapse = "|")) %>% 
    dplyr::select(dplyr::all_of(c(!!patient_id, 'sample_string'))) %>% 
    dplyr::slice(1)
  
  clinical_data_clean <- clinical_data %>% 
    dplyr::full_join(sample_data_clean, by = patient_id) %>%
    dplyr::select(dplyr::all_of(c(!!patient_id, 'sample_string')), dplyr::all_of(dplyr::everything()))
  
  
  if(!is.null(spatial_list) & is.null(names(spatial_list))){
    
    spatial_names <- lapply(spatial_list, function(x) {x[[sample_id]][[1]]})
    spatial_names <- unlist(spatial_names)
    
    names(spatial_list) <- spatial_names
    
  }
  
  if(is.null(spatial_list)) {
    spatial_list <- list(NA)
  }

  mif <- list(clinical = clinical_data,
              sample = sample_data,
              spatial = spatial_list,
              derived = list(),
              patient_id = patient_id,
              sample_id = sample_id)
  
  structure(mif, class="mif")
  
  # return(mif)

}