Description Usage Arguments Value Examples
eds.summary
1 | eds.summary(data, Experimental_conditions = NULL, Problems = NULL)
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data |
This is a dataframe created by the read.sds function |
Experimental_conditions |
If you have multiple experimental conditions that you want to compare this is a character vector of those conditions. By default it is NULL |
Problems |
This is a character vector of the wells (specified by coordinate e.g. "A1") which have been identified as problematic by looking at the Ct curves etc. They get removed from further analysis |
This function takes the eds data and does the munging to get it in a useful form for your experiment, especially if multiple experiments are done on one plate. Essentially it averages out technical replicates for a given experiment and groups biological replicates of the same measurements for downstram analysis. It returns a list with each entry being a dataframe for a gene/condition combination.
1 2 3 | Experimental_conditions <- c("SD", "LD")
Problems <- c("A14", "B1", "B12", "I4", "I5", "I6", "J1")
eds.summary(test, Experimental_conditions = Experimental_conditions, Problems = Problems)
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