R/MEDIPS.createSet.R

In HPCBio/MEDIPS-BioC: DNA IP-seq data analysis

##########################################################################
##Creates MEDIPS SET of type S4 from input data (i.e. aligned short reads)
##########################################################################
##Input:	bam file or tab (|) separated txt file "chr | start | stop  | strand"
##Param:	[path]+file name, extend, shift, window_size, BSgenome, uniq, chr.select
##Output:	MEDIPS SET
##Requires:	gtools, BSgenome
##Modified:	22/4/2015
##Author:	Lukas Chavez, Matthias Lienhard, Isaac Lopez Moyado

MEDIPS.createSet <-
function (file = NULL, extend = 0, shift = 0, window_size = 300, 
    BSgenome = NULL, uniq = 1e-3, chr.select = NULL, paired = F, 
    sample_name = NULL, isSecondaryAlignment = FALSE, simpleCigar=TRUE) 
{
    if (is.null(BSgenome)) {
        stop("Must specify a BSgenome library.")
    }
    if (is.null(file)) {
        stop("Must specify a bam or txt file.")
    }
    fileName = unlist(strsplit(file, "/"))[length(unlist(strsplit(file, 
        "/")))]
    path = paste(unlist(strsplit(file, "/"))[1:(length(unlist(strsplit(file, 
        "/")))) - 1], collapse = "/")
    if (path == "") {
        path = getwd()
    }
    dataset = get(ls(paste("package:", BSgenome, sep = "")))
    if (is.null(chr.select)) {
        cat("All chromosomes in the reference BSgenome will be processed:\n")
        chr.select = seqnames(dataset)
        print(chr.select)
    }
    else {
        if (sum(!chr.select %in% seqnames(dataset)) != 0) {
            cat("The requested chromosome(s)", chr.select[!chr.select %in% 
                seqnames(dataset)], "are not available in the BSgenome reference. Chromosomes available in the BSgenome reference:", 
                seqnames(dataset), "\n")
            stop("Please check chromosome names.")
        }
    }
    if (length(chr.select) > 1) {
        chr.select = mixedsort(unique(chr.select))
    }
    if (!fileName %in% dir(path)) {
        stop(paste("File", fileName, " not found in", path, sep = " "))
    }
    ext = strsplit(fileName, ".", fixed = T)[[1]]
    if (ext[length(ext)] %in% c("gz", "zip", "bz2")) 
        ext = ext[-length(ext)]
    if (ext[length(ext)] %in% c("wig", "bw", "bigwig")) {
        MEDIPSsetObj = getMObjectFromWIG(fileName, path, chr.select, 
            BSgenome)
    }
    else {
        if (!paired) {
            GRange.Reads = getGRange(fileName, path, extend, 
                shift, chr.select, dataset, uniq, isSecondaryAlignment = isSecondaryAlignment, simpleCigar=simpleCigar)
        }
        else {
            GRange.Reads = getPairedGRange(fileName, path, extend, 
                shift, chr.select, dataset, uniq, isSecondaryAlignment = isSecondaryAlignment, simpleCigar=simpleCigar)
        }
        chr_lengths = as.numeric(seqlengths(dataset)[chr.select])
        no_chr_windows = ceiling(chr_lengths/window_size)
        supersize_chr = cumsum(no_chr_windows)
        Granges.genomeVec = MEDIPS.GenomicCoordinates(supersize_chr, 
            no_chr_windows, chr.select, chr_lengths, window_size)
        
        cat("Calculating short read coverage at genome wide windows...\n")
        overlap = countOverlaps(Granges.genomeVec, GRange.Reads)
        datachr = unique(seqlevels(GRange.Reads))
       
        if (sum(!chr.select %in% datachr) != 0) {
            cat("Please note, no data in the alignment file for chromosome(s):", 
                chr.select[!chr.select %in% datachr], "\n")
        }
       
        if (is.null(sample_name)) {
            sample_name = fileName
        }

        MEDIPSsetObj = new("MEDIPSset", sample_name = sample_name, 
            path_name = path, genome_name = BSgenome, number_regions = length(GRange.Reads), 
            chr_names = chr.select, chr_lengths = chr_lengths, 
            genome_count = overlap, extend = extend, shifted = shift, 
            window_size = window_size, uniq = uniq)
    }
    return(MEDIPSsetObj)
}