LabTranslationalArchitectomics/riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data

riboWaltz is an R package designed for the analysis of ribosome profiling (RiboSeq) data aimed at the identification of the P-site offset. The P-site offset (PO) is specified by the localization of the P-site of ribosomes within the fragments of the RNA (reads) resulting from RiboSeq assays. It is defined as the distance of the P-site from the two ends of the reads. Determining the PO is a crucial step for a variety of RiboSeq-based analyses such as verify the so-called 3-nt periodicity of ribosomes along the coding sequence, derive translation initiation and elongation rates and reveal new translational events in unannotated open reading frames and ncRNAs. riboWaltz performs accurate computation of the PO for all the lengths of reads from single or multiple samples, taking advantage from an original two-step algorithm. Moreover, riboWaltz provides the user a variety of graphical representations, laying the groundwork for further positional analyses and new biological discoveries.

install.packages("remotes")
remotes::install_github("LabTranslationalArchitectomics/riboWaltz")