Man pages for NPhogat/RTqPCR
A work flow for analysis and normalization of pre-processing RT-qPCR data

CombineTechRepsCombine technical replicates of Cq and amplification...
CqEffsCompute Cq values and amplification efficiencies
DeltaCqCompute delta Cq value and standard deviation of delta Cq
DeltaDeltaCqCompute delta delta Cq, standard deviation of delta delta Cq...
DeltaDeltaCqAllCompute delta delta Cq, standard deviation of delta delta Cq...
NonDetectsRemove the non-detects ('NA') of Cq and amplification...
NRQeffsCompute relative expression ratio
NRQeffsAllCompute normalized relative expression of all genes at one...
Read.LC480SampleInfoRead sample information file of raw data of LC480 RT-qPCR
read.Mx3005PTo read in the raw fluorescent data of Mx3005P (Agilent)...
Read.Mx3005PSampleInfoRead sample information file of raw data of Mx3005P RT-qPCR
read.RTqPCRTo read in the raw fluorescent data of LC480 light cycler and...
read.RTqPCRSampleInfoRead sample information file of raw data of RT-qPCRs (LC480...
ReplaceAboveCutOffReplace the Cq and amplification efficiency above cut off by...
ReplaceNAsReplace the all the non detects of Cq and amplification...
ReplaceValueReplace the specific Cq value and amplification efficiency by...
RTqPCRBatch-classClass RTqPCRBatch
RTqPCR.dataframeConvert the object of Class '"data.frame"' into an object of...
RTqPCR.guiRun the graphical User Interface
RTqPCR-packageWork Flow of RT-qPCR
NPhogat/RTqPCR documentation built on July 12, 2020, 12:56 p.m.