NRQeffsAll: Compute normalized relative expression of all genes at one...

In NPhogat/RTqPCR: A work flow for analysis and normalization of pre-processing RT-qPCR data

Description

Compute relative expression ration of all genes at one time of an object of "RTqPCRBatch".

Usage

## S4 method for signature 'RTqPCRBatch'
NRQeffsAll(RTqPCRBatch, y, ...)

Arguments

RTqPCRBatch	An object of class "RTqPCRBatch" produced as an output of CombineTechReps function.
y	Name of Reference gene under column Target name.
...	other parameters to be passed to the downstream methods.

Value

An object of "RTqPCRBatch" with new slots.

Author(s)

Navneet Phogat, Matthias Kohl, [e-mail]

Examples

## Read in the raw fluorescent data

## Read in the sample information data

## Merge the fluorescence and sample information data through merge function

## Compute the Cq values and amplification efficiencies

##  Implement the functions ReplaceNAs, ReplaceAboveCutOff, ReplaceValue and NonDetects as per requirement.

## Combine technical replicates - on the basis of efficiency

effsreps <- CombineTechReps(Cqeffs.LC480, cRepCq = FALSE)

##To compute the relative expression ratio
nrqeffsall <- NRQeffsAll(effsreps, y = "hk")
nrqeffsall

NPhogat/RTqPCR documentation built on July 12, 2020, 12:56 p.m.