DeltaDeltaCqAll: Compute delta delta Cq, standard deviation of delta delta Cq...

In NPhogat/RTqPCR: A work flow for analysis and normalization of pre-processing RT-qPCR data

Description

Compute delta delta Cq, standard deviation of delta delta Cq and fold concentration of an object of "RTqPCRBatch".

Usage

## S4 method for signature 'RTqPCRBatch'
DeltaDeltaCqAll(RTqPCRBatch, ...)

Arguments

...	other parameters to be passed to the downstream methods.
x	An object of class "RTqPCRBatch" produced as an output of DeltaCq function.

Value

An object of "RTqPCRBatch" with new slots.

Author(s)

Navneet Phogat, Matthias Kohl, [e-mail] #'@examples

## Read in the raw fluorescent data

## Read in the sample information data

## Merge the fluorescence and sample information data through merge function

## Compute the Cq values and amplification efficiencies

## Implement the functions ReplaceNAs, ReplaceAboveCutOff, ReplaceValue and NonDetects as per requirement.

## Combine technical replicates (based on Cq values) : see also CombineTechReps function and vignettes

Cqreps <- CombineTechReps(Cqeffs.LC480) Cqreps ## to visualise the overview of the resulting data

## To compute the delta Cq value deltaCq <- DeltaCq(Cqreps, Ref = "hk") deltaCq

## For more details see the vignettes deltadeltaCqall <- DeltaDeltaCqAll(deltaCq) deltadeltaCqall

NPhogat/RTqPCR documentation built on July 12, 2020, 12:56 p.m.