recovery_advise_lipidomics: recovery_advise_lipidomics

In ShinyFabio/ADViSELipidomics: Shinyapp for the acquisition and the analysis of lipidomics data

recovery_advise_lipidomics

Description

recovery_advise_lipidomics is the function for the computation of the recovery percentages, and their application to define the concentration matrix (i.e. feature matrix)

Usage

recovery_advise_lipidomics(out, intercept_flag = TRUE)

Arguments

out	List. It is the result from the calibplot_advise_lipidomics function.
intercept_flag	Logical value. If set to TRUE the intercept will be set to zero.

Details

The recovery step is the computation of the recovery percentage for each internal standard lipid species, and the application of the recovery percentage on all the filter lipid species (non-labeled species). The complete algorithm of the computation and the application of the recovery percentage is composed of two main steps: a) the recovery percentage is calculated by the formula (q intercept, m slope, NominalStdConcentration is a value of concentration from the internal standard file): - (Area/m)*100/NominalStdConcentration for intercept as zero; - (Area-q)/m)*100/NominalStdConcentration for intercept as not zero; b) the recovery percentage is applied to the lipid species by the formula (ConcExpPerc is the recovery percentage): - (Area/m)*100.00/ConcExpPerc for intercept as zero; - (Area-q)/m)*100.00/ConcExpPerc for intercept as not zero.

Value

res: a list with results from calibration steps, updated with the recovery percentages and the concentration matrix (feature matrix).

Note

Last change 14/01/2022

ShinyFabio/ADViSELipidomics documentation built on March 21, 2023, 7:30 a.m.