get_df_wide: Generate a wide data.frame from a SummarizedExperiment

Description Usage Arguments Value Examples

View source: R/functions.R

Description

get_df_wide generate a wide data.frame from a SummarizedExperiment.

Usage

1

Arguments

se

SummarizedExperiment, Proteomics data (output from make_se() or make_se_parse()).

Value

A data.frame object containing all data in a wide format, where each row represents a protein.

Examples

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# Load example
data <- UbiLength
data <- data[data$Reverse != "+" & data$Potential.contaminant != "+",]
data_unique <- make_unique(data, "Gene.names", "Protein.IDs", delim = ";")

# Make SummarizedExperiment
columns <- grep("LFQ.", colnames(data_unique))
exp_design <- UbiLength_ExpDesign
se <- make_se(data_unique, columns, exp_design)

# Filter, normalize and impute missing values
filt <- filter_missval(se, thr = 0)
norm <- normalize_vsn(filt)
imputed <- impute(norm, fun = "MinProb", q = 0.01)

# Test for differentially expressed proteins
diff <- test_diff(imputed, "control", "Ctrl")
dep <- add_rejections(diff, alpha = 0.05, lfc = 1)

# Get a wide data.frame
wide <- get_df_wide(dep)
colnames(wide)

arnesmits/DEP documentation built on June 14, 2018, 7:24 p.m.