bjreisman/DebarcodeR: Automated Demultiplexing for Fluorescent Cell Barcoding

DebarcodeR is an R package for demultiplexing fluorescent cell barcoded (FCB) barcoded flow cytometery data. Fluorescent cell barcoding is a technique for implementing pooled sample multiplexing in fluorescence flow cytometry using amine reactive dyes to label each sample with unique barcodes by varying the concentration of one or more dyes. Sample can the be pooled, stained, and acquired in a single tube, which reduces instrument time and reagent consumption and improves data robustness. Following acquisition, the data must be 'debarcoded' (also known as deconvolution or demultiplexing) which traditionally has required manual biaxial gating. DebarcodeR implements an algorithm for automating this demultiplexing process which improves reproducibility and enables high throughput data processing. For more information about DebarcodeR, see our manuscript and for more information about FCB, see Krutzik et al. 2006.

install.packages("remotes")
remotes::install_github("bjreisman/DebarcodeR")