View source: R/deskew_fcbFlowSet.R
deskew_fcbFlowSet | R Documentation |
Corrects morphology based on scatter or uptake control for fcbFlowFrame
deskew_fcbFlowSet( fcbFlowSet, uptake = NULL, channel, method = "earth", predictors = c("fsc_a", "ssc_a"), subsample = 20000, ret.model = TRUE, verbose = FALSE, updateProgress = NULL, ... )
channel |
The name (string) of the channel to be corrected, ie. the column name in fcbFlowFrame barcoded.ff exprs |
method |
The name of the morphology correction model to use. Choose between earth, lm (linear model), or knijnenburg |
predictors |
The vector of channel names to be used to build the regression model |
subsample |
Integer, number of cells to sample (with replacement) for the morphology correction, defaults to 10,000. |
ret.model |
Option to retain the model for deskewing |
updateProgress |
used in reactive context (shiny) to return progress information to GUI |
fcbFlowFrame |
a fcbFlowFrame object with barcoded flowframe and uptake flowframe, post compensation and preprocessing |
a fcbFlowFrame with barcode slots added for selected channel corrected for predictors chosen
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