assign_fcbFlowSet: Defines populations on barcoded datasets

In bjreisman/DebarcodeR: Automated Demultiplexing for Fluorescent Cell Barcoding

Defines populations on barcoded datasets

Description

Defines populations on barcoded datasets

Usage

assign_fcbFlowSet(fcbFlowSet, channel, likelihoodcut = 8, ambiguitycut = 0.02)

Arguments

channel	The name (string) of the channel that has been corrected and clustered
likelihoodcut	numeric, a likelihood cutoff for discarding unlikely cells, less than 1/k as likely as the most likely cell from that population
ambiguitycut	numeric from 0 to 1, threshhold below which to discard ambigious cells, eg: 0.02, discards cells with more than 2% chance of originating from another population
fcbFlowFrame	a fcbFlowFrame object with barcoded flowframe and uptake flowframe post deskewing and clustering (at least one barcodes slot filled)

Value

a fcbFlowFrame object with a barcode slot filled with deskewing, clustering, cell assignment as a vector of integers from 0:ncol(probs), cells assigned a classification of 0 remained unassigned, otherwise number corresponds to the barcoding level assignment of that cell

bjreisman/DebarcodeR documentation built on Oct. 22, 2022, 1:50 a.m.