Bulk_Read_Counts: Parekh et al. 2016: Gene Expression Matrices
In bvieth/powsimR: Power Simulations for RNA-sequencing
Bulk_Read_Counts R Documentation
Parekh et al. 2016: Gene Expression Matrices
Description
250 ng of Universal Human Reference RNA (UHRR; Agilent Technologies; catalog #740000) and ERCC spike-in control mix I (Life Technologies) were used and cDNA was synthesized as described in the Smart-Seq2 protocol from Picelli et al. 2013.
Base-calls were performed with Illumina bcl2fastq.
All cDNA reads were aligned with NextGenMap 0.4.12. Smart-seq2 reads were mapped to GRCh37 (hg19) and ERCC synthetic spike-in reference.
Reads were assigned to genes using FeatureCount method in Rsubread package.
Usage
data(Bulk_Read_Counts)
Format
An object of class data.frame with 57773 rows and 10 columns.
Source
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75823.
bvieth/powsimR documentation built on Aug. 19, 2023, 7:48 p.m.
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| Bulk_Read_Counts | R Documentation |
Parekh et al. 2016: Gene Expression Matrices
Description
250 ng of Universal Human Reference RNA (UHRR; Agilent Technologies; catalog #740000) and ERCC spike-in control mix I (Life Technologies) were used and cDNA was synthesized as described in the Smart-Seq2 protocol from Picelli et al. 2013.
Base-calls were performed with Illumina bcl2fastq.
All cDNA reads were aligned with NextGenMap 0.4.12. Smart-seq2 reads were mapped to GRCh37 (hg19) and ERCC synthetic spike-in reference.
Reads were assigned to genes using FeatureCount method in Rsubread package.
Usage
data(Bulk_Read_Counts)
Format
An object of class data.frame with 57773 rows and 10 columns.
Source
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75823.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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