breakTranscriptsOnGenes: breakTranscriptsOnGenes Breaks transcripts on genes

In coregenomics/groHMM: GRO-seq Analysis Pipeline

Description

Breaks transcripts when they are overlapped with multiple well annotated genes.

Usage

breakTranscriptsOnGenes(tx, annox, strand = "+", geneSize = 5000,
  threshold = 0.8, gap = 5, plot = FALSE)

Arguments

tx	GRanges of transcripts.
annox	GRanges of non-overlapping annotations for reference.
strand	Takes "+" or "-" Default: "+"
geneSize	Numeric. Minimum gene size in annox to be used as reference. Default: 5000
threshold	Numeric. Ratio of overlapped region relative to a gene width. Transcripts only greater than this threshold are subjected to be broken. Default: 0.8
gap	Numeric. Gap (bp) between broken transcripts. Default: 5
plot	Logical. If set to TRUE, show each step in a plot. Default: FALSE

Value

Returns GRanges object of broken transcripts.

Author(s)

Minho Chae and Charles G. Danko

Examples

library(GenomicRanges)
tx <- GRanges("chr7", IRanges(1000, 30000), strand="+")
annox <- GRanges(
    "chr7", IRanges(start=c(1000, 20000), width=c(10000,10000)), strand="+")
bPlus <- breakTranscriptsOnGenes(tx, annox, strand="+")

coregenomics/groHMM documentation built on May 7, 2019, 7:57 a.m.