nc_ratio_tiff: Determine Nucleus to Cytoplasm Signal Ratio
In dami82/CellSignalingTools: Analysis of Images for Molecular Biology Applications

Description Usage Arguments Value Note Author(s) References Examples

Determine the ratio of Nuclear to non-nuclear Signal intensity from processed stacked TIFF images. It also plots a graphic representation of nuclear and non-nuclear signal.

1	nc_ratio_tiff(signal_data, signal_cutoff = 0)

`signal_data`	is a list containing 3 numeric matrices of identical dimensions. Typically, this is the result of a apply_mask() call.
`signal_cutoff`	a number in the range 0 to 1.

It returns a number corresponding to the ratio between nuclear and non-nuclear signal.

It is advisable not to set a signal cutoff.

Damiano Fantini

http://www.biotechworld.it/bioinf/2016/03/09/analyzing-fluoresence-microscopy-data-with-r/

data("leio_cells_dapi")
data("leio_cells_green")
dapi_mask <- get_dapi_range(leio_cells_dapi, 0.05)
d_msk <- dapi_mask[500:1100, 700:1100]
g_img <- leio_cells_green[500:1100, 700:1100] 
nc_signal <- apply_mask(g_img, d_msk, bckgrnd = 0.05)
nc_ratio_tiff(nc_signal)