leio_pq100_profile: Immunofluorescence Signal Profiles of Paraquat Stressed...

In dami82/CellSignalingTools: Analysis of Images for Molecular Biology Applications

Description

Position and Signal Intensity Values of a single cell from an Immunofluorescence experiment performed on Leiomyoma cells treated with Paraquat. Cells were immunostained with an anti-PTEN-Alexa488 antibody and then counterstained with DAPI. Stacked 8-bit images corresponding to the blue (B1) and the green (G1) channels were analyzed using the "line select" and the "plot profile" tools of ImageJ.

Usage

data("leio_pq100_profile")

Format

A data frame with 489 observations on the following 3 variables.

X1

a numeric vector; contains position information along x-axis

G1

a numeric vector; contains values of signal intensities from the green channel

B1

a numeric vector; contains values of signal intensities from the DAPI channel

Details

Leiomyoma Cells were treated with Paraquat for 6 hours and then fixed with PFA. Fixed samples were stained with an anti-PTEN primary antibody and then counterstained with DAPI. For further information, please check the reference cited below. This dataset is part of an experiment including a mock-treated sample as well ("leio_basal_profile").

Source

Vidimar V et al, 2016? (in preparation)

References

http://www.biotechworld.it/bioinf/2016/03/09/analyzing-fluoresence-microscopy-data-with-r/

Examples

data(leio_pq100_profile)
with(leio_pq100_profile, plot(B1~X1, pch = 16, cex = 0.75))
with(leio_pq100_profile, lines(G1~X1, col = "green", lwd = 3))

dami82/CellSignalingTools documentation built on May 14, 2019, 3:32 p.m.