remove_dust: Clean and Adjust Signal of a DAPI Mask

Description Usage Arguments Value Author(s) References Examples

Description

Clean and Adjust Signal of a DAPI Mask. Voids in large signal areas are filled while small signal areas (aspecific signal) are removed.

Usage

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remove_dust(signal_map, nuclei_dims)

Arguments

signal_map

is a boolean matrix. Typically, this is the result of a get_dapi_range() call.

nuclei_dims

is an integer vector containing two elements. Typically, this is the result of a get_nucl_dims() call.

Value

The function returns a boolean matrix

Author(s)

Damiano Fantini

References

http://www.biotechworld.it/bioinf/2016/03/09/analyzing-fluoresence-microscopy-data-with-r/

Examples

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data("leio_cells_dapi")
dapi_mask <- get_dapi_range(leio_cells_dapi)
n_dims <- get_nucl_dims(dapi_mask)
clean_mask <- remove_dust(dapi_mask[300:800,400:800], n_dims)
image(clean_mask, col = c("gray75", "white"))

dami82/CellSignalingTools documentation built on May 14, 2019, 3:32 p.m.