Description Usage Arguments Value Author(s) References Examples
Clean and Adjust Signal of a DAPI Mask. Voids in large signal areas are filled while small signal areas (aspecific signal) are removed.
1 | remove_dust(signal_map, nuclei_dims)
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signal_map |
is a boolean matrix. Typically, this is the result of a get_dapi_range() call. |
nuclei_dims |
is an integer vector containing two elements. Typically, this is the result of a get_nucl_dims() call. |
The function returns a boolean matrix
Damiano Fantini
http://www.biotechworld.it/bioinf/2016/03/09/analyzing-fluoresence-microscopy-data-with-r/
1 2 3 4 5 | data("leio_cells_dapi")
dapi_mask <- get_dapi_range(leio_cells_dapi)
n_dims <- get_nucl_dims(dapi_mask)
clean_mask <- remove_dust(dapi_mask[300:800,400:800], n_dims)
image(clean_mask, col = c("gray75", "white"))
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