dct: Calculation of dCt values

In dcorujog/qpcr-package: Analysis of qPCR data with the delta Ct method

Calculation of dCt values

Description

Function to calculate dCt values according to primer efficiency and a calibrator sample

Usage

dct(x, calib, eff.matrix)

Arguments

x	a row of data from the averaged qPCR data as generated by ct_avg_fun, effectively containing the averaged data for a single sample
calib	a character string with the name of the sample to be used as reference for dct calculation. This sample will have an expression value of 1 for all targets and the rest of the samples will have values expressing their relative expression to this sample.
eff.matrix	a data.frame containing the efficiency values for each primer pair as generated in qpcr_analysis

Value

the dCt value calculated for the row of data

dcorujog/qpcr-package documentation built on Sept. 4, 2023, 3:04 p.m.