norm_factor_fun: Calculate normalization factor

In dcorujog/qpcr-package: Analysis of qPCR data with the delta Ct method

Calculate normalization factor

Description

Calculate a normalization factor for each sample using the average of the dct values of all genes used for normalization.

Usage

norm_factor_fun(dct_data, hkg, exp_name)

Arguments

dct_data	data.frame containing the qPCR data including dCt values as calculated by dct_apply
hkg	a character vector containing the names of the targets used as normalization genes
exp_name	experiment name (used as plot title and to name the saved file)

Value

a named numeric vector with the normalization factor calculated for each sample. In addition, saves a PDF containing a scatterplot to asses the correlation between the expression values of normalization genes (if using more than one)

dcorujog/qpcr-package documentation built on Sept. 4, 2023, 3:04 p.m.