dct_apply: Apply dCt calculation over rows

In dcorujog/qpcr-package: Analysis of qPCR data with the delta Ct method

Apply dCt calculation over rows

Description

A helper function to apply the dct function to each row of the data.frame containing the qPCR data

Usage

dct_apply(data, calibsample, eff)

Arguments

data	a data.frame containing the qPCR data with averaged technical replicates as generated by ct_avg_fun.
calibsample	a character string with the name of the sample to be used as reference for dct calculation. This sample will have an expression value of 1 for all targets and the rest of the samples will have values expressing their relative expression to this sample.
eff	a data.frame containing the efficiency values for each primer pair as generated in qpcr_analysis

Value

a data.frame containing the qPCR data with an additional column containing the dCt values

dcorujog/qpcr-package documentation built on Sept. 4, 2023, 3:04 p.m.