checkPrimer: checkPrimer

View source: R/primerpcr.R

checkPrimerR Documentation

checkPrimer

Description

checkPrimer

Usage

checkPrimer(pp, genome, roi = NULL)

Arguments

pp

data.frame defining primers, or output of callPrimer3. minimal columns = PRIMER_LEFT_SEQUENCE,PRIMER_RIGHT_SEQUENCE

genome

BSgenome object

roi

makeROI object

Value

list of GRanges with primer locations

Author(s)

Diana Low

Examples

# create a primer pair
roi
primer_pair <- data.frame(PRIMER_LEFT_SEQUENCE="agctcttgaaattggagctgac",
                          PRIMER_RIGHT_SEQUENCE="cttagaaagaacaggaaatcc",
                          stringsAsFactors=FALSE)

dianalow/SPLINTER documentation built on March 28, 2024, 2:07 p.m.