addGRLgaps: Add gaps between GRangesList regions

In jmw86069/splicejam: Analysis and Visualization of Gene Splice Variants and Transcriptome Data

Add gaps between GRangesList regions

Description

Add gaps between GRangesList regions

Usage

addGRLgaps(
  grl,
  strandSpecific = TRUE,
  gapname = "gap",
  suffix = "_v",
  newValues = list(feature_type = "gap"),
  default_feature_type = "exon",
  feature_type_colname = "feature_type",
  doSort = TRUE,
  verbose = FALSE,
  ...
)

Arguments

grl	GRangesList object
strandSpecific	logical indicating whether the gaps are calculated per strand, see getGRLgaps().
gapname, suffix	character vector supplying the name to assign to new gap GRanges elements, using jamba::makeNames() with suffix as described to define non-duplicated names. If ⁠gapname is NULL⁠ then no names are assigned to new gap GRanges entries, however when the input gr GRanges object has names, the concatenation of gaps causes names "" to be assigned to all gap GRanges elements, which are duplicated for multiple gaps.
newValues	list of values to add to the resulting gap GRanges, whose names become colnames(grl@unlistData), and whose values are used to populate each column. By default a colname "feature_type" is added, with value "gap" added to each row. When newValues is NULL then no values are added to the gaps GRanges. For every entry in names(newValues), if the colname does not exist, it is created and populated with default_feature_type as a default value, prior to adding gaps.
default_feature_type, feature_type_colname	character values, indicating the type and colname to populate in values(grl@unlistData) prior to adding gaps. When feature_type_colname is NULL, no action is taken. When feature_type_colname does not exist in colnames(values(grl@unlistData)), it is created with values in default_feature_type. Also any colname defined by newValues that does not already exist is also created and populated with default_feature_type.
doSort	logical indicating whether to sort the resulting GRanges objects. When doSort=FALSE the gaps are added to the end of each input grl GRanges object. Note that the GrangesList object is not sorted, only the GRanges objects within the GRangesList are sorted.
verbose	logical indicating whether to print verbose output.
...	additional arguments are passed to getGRLgaps().

Details

This function adds gaps between each GRanges region separately for each GRangesList element, where there is a gap between two GRanges for the same seqnames. When strandSpecific=TRUE the gaps are determined per strand.

This function is a wrapper around getGRLgaps(), which is then concatenated to the input gr GRanges object using S4Vectors::pc(). When the input grl GRanges has column S4Vectors::values() then the gaps GRanges object will have NA values used by default. To supply values, use the newValues argument, which assigns name-value pairs.

Value

GRangesList object, sorted per GRangesList element when doSort=TRUE. When newValues is supplied, the values for gaps GRanges elements will be assigned, otherwise any column values present in gr will be NA for gaps elements. The names of gaps elements are assigned using gapname then are made unique using jamba::makeNames(), unless ⁠gapname is NULL⁠.

See Also

Other jam GRanges functions: addGRgaps(), annotateGRLfromGRL(), annotateGRfromGR(), assignGRLexonNames(), closestExonToJunctions(), combineGRcoverage(), exoncov2polygon(), findOverlapsGRL(), flattenExonsBy(), getFirstStrandedFromGRL(), getGRLgaps(), getGRcoverageFromBw(), getGRgaps(), grl2df(), jam_isDisjoint(), make_ref2compressed(), sortGRL(), spliceGR2junctionDF(), stackJunctions()

Examples

gr <- GenomicRanges::GRanges(seqnames=rep(c("chr1","chr2"), c(3,2)),
   ranges=IRanges::IRanges(start=c(100, 300, 400, 300, 700),
      end=c(199, 450, 500, 600, 800)),
   strand=rep(c("+","-"), c(3,2)),
   feature_type=rep("exon", 5));
names(gr) <- jamba::makeNames(rep("exon", length(gr)));
gr;
addGRgaps(gr);

grl <- GenomicRanges::split(gr, GenomicRanges::seqnames(gr));
grl;
addGRLgaps(grl);
addGRLgaps(grl, strandSpecific=FALSE);

jmw86069/splicejam documentation built on April 21, 2024, 4:57 p.m.