internal_junc_score: maximum overlapping internal junction score

In jmw86069/splicejam: Analysis and Visualization of Gene Splice Variants and Transcriptome Data

maximum overlapping internal junction score

Description

maximum overlapping internal junction score

Usage

internal_junc_score(
  juncGR,
  scoreColname = "score",
  sampleColname = "sample_id",
  minScore = 0,
  verbose = FALSE,
  ...
)

Arguments

juncGR	GRanges containing splice junctions, with numeric column scoreColname representing the abundance of splice junction-spanning sequence reads.
scoreColname, sampleColname	colnames in values(juncGR) which define the junction score, and the "sample_id".
minScore	optional numeric value indicating the minimum score to use, which can be useful for example if there is no matching scoreColname. Note that values are matched using absolute value, but the original sign is maintained.
verbose	logical indicating whether to print verbose output.
...	additional arguments are ignored.

Details

This function is intended for internal use, and calculates the maximum score for junction GRanges for the special case where:

• a junction range overlaps the start or end of another junction

• the start or end of the other junction is internal, that is it does not overlap the same start or end.

• overlaps are constrained to the same "sample_id" stored in values(juncGR)$sample_id.

The goal is to determine the highest score contained inside each junction region, so that the junction arc height can be defined higher in order to minimize junction arc overlaps.

Value

numeric vector named by names(juncGR) whose values are the maximum score of internal overlapping junction ends.

See Also

Other jam RNA-seq functions: assignGRLexonNames(), closestExonToJunctions(), combineGRcoverage(), defineDetectedTx(), detectedTxInfo(), exoncov2polygon(), flattenExonsBy(), getGRcoverageFromBw(), groups2contrasts(), makeTx2geneFromGtf(), make_ref2compressed(), prepareSashimi(), runDiffSplice(), sortSamples(), spliceGR2junctionDF()

jmw86069/splicejam documentation built on Nov. 4, 2024, 10:53 a.m.