This function normalizes the data by using NormalizeData() of Seurat package with the argument normalization.method = LogNormalize, (2) performs variance stabilizing transform (VST) by using FindVariableFeatures(), setting the number of variable feature nfeatures, (3) scales the data, and (4) reduce the dimension by principal component analysis (PCA), using RunPCA() with the argument: features = VariableFeatures(obj)
1 | process_001_seurat(obj, nfeatures)
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obj |
Seurat object |
nfeatures |
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