specUnmix: Spectral unmixing of cytometry files
In jtheorell/theFlowSpec: Tools for spectral unmixing of overdetermined flow-cytometry files. And other nice things.
Description
Usage
Arguments
Value
Examples
Description
This function performs the central task of spectral unmixing, to convert the
photon input to the detectors to biological proxy-signals.
Usage
1
Arguments
flowObj
The fcs object to be filtered. Both flowFrames and flowSets
are accepted.
specMat
This is a matrix generated by the secMatCalc function,
possibly with edited row names.
Value
The unmixed data. It will be returned in the format it was imported as.
Examples
1
2
3
4
5
6
7
8
9
# Load some uncompensated data
# Load the spectral unmixing matrix generated with controls from the same
experiment. These can be generated using the specMatCalc function.
data(specMat)
# And now, just run the function!
dataComp <- specUnmix(fluoExprs, specMat)
jtheorell/theFlowSpec documentation built on Aug. 22, 2019, 3:33 a.m.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Description Usage Arguments Value Examples
Description
This function performs the central task of spectral unmixing, to convert the photon input to the detectors to biological proxy-signals.
Usage
1 |
Arguments
flowObj |
The fcs object to be filtered. Both flowFrames and flowSets are accepted. |
specMat |
This is a matrix generated by the secMatCalc function, possibly with edited row names. |
Value
The unmixed data. It will be returned in the format it was imported as.
Examples
1 2 3 4 5 6 7 8 9 | # Load some uncompensated data
# Load the spectral unmixing matrix generated with controls from the same
experiment. These can be generated using the specMatCalc function.
data(specMat)
# And now, just run the function!
dataComp <- specUnmix(fluoExprs, specMat)
|
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
Embedding an R snippet on your website
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.