R/data.pacific_oyster_18S_V4.R

In kevinzhongxu/CasOligo: A R package to search the gRNA-target-site oligo for designing the taxon-specific guide RNA used for CRISPR-Cas Selective Amplicon Sequencing (CCSAS) to assess the eukaryotic microbiome of hosts (e.g. metazoans, plant)

#' pacific_oyster_18S_V4:
#'
#' 18S rRNA gene sequence of pacific oyster at V4 region that amplified by primers "TAReuk454FWD1 and TAReukREV3" (Stoeck et al., 2010).
#' This serves as an example of input fasta file for cas9.gRNA.oligo1, cas9.gRNA.oligo2, cas12a.gRNA.oligo1 and cas12a.gRNA.oligo2.
#'
#' @docType data
#'
#' @usage data(pacific_oyster_18S_V4)
#'
#' @format A data frame
#'
#' @keywords pacific_oyster_18S_V4
#'
#' @references Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA. (2010)
#' Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water
#' Mol. Ecol. 19, 21–31.
#' (\href{https://www.ncbi.nlm.nih.gov/pubmed/20331767}{PubMed})
#'
#' @examples
#' data(pacific_oyster_18S_V4)

"pacific_oyster_18S_V4"