Description Usage Arguments Value Note Author(s) Examples
Simply point to the final upload directory output by bcbio-nextgen, and this function will take care of the rest. It automatically imports small RNA-seq counts, metadata, and program versions used.
1 2 3 | loadSmallRnaRun(projectDir = "date-final",
interestingGroups = "sample", maxSamples = 50, minHits = 5,
dataDir = NULL, colData = NULL, ...)
|
projectDir |
Path to final upload directory. This path is set when
running |
interestingGroups |
Character vector of interesting groups. First entry is used for plot colors during quality control (QC) analysis. Entire vector is used for PCA and heatmap QC functions. |
maxSamples |
Optional. Maximum number of samples to calculate rlog and variance stabilization object from DESeq2. |
minHits |
Optional. Minimum lines to have in the miRNA output to load the sample. |
dataDir |
Folder to keep a cache of the object. |
colData |
Optional External metadata to be used while reading samples. |
... |
Additional arguments, saved as metadata. |
bcbioSmallRnaDataSet.
When working in RStudio, we recommend connecting to the bcbio-nextgen run directory as a remote connection over sshfs.
Michael Steinbaugh, Lorena Pantano
1 2 3 | path <- system.file("extra", package="bcbioSmallRna")
sbcb <- loadSmallRnaRun(file.path(path, "geu_tiny", "final",
"2018-12-05_geu_tiny"), "population")
|
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