Description Usage Arguments Value
This function calculates the minimum sample size required for your assay with varying paremeters, such as simulated effect size and TypeI/TypeII error rates. This can be completed using .XML or .CSV file formats.
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Pos.file |
A .xml file derived from your positive control condition, i.e., that with the highest value. |
Neg.file |
A .xml file derived from your negative control condition, i.e., that with the lowest value. |
Roy.file |
A .xml file of raw signal values from a 96-well plate of non-transgenic Roy larvae. |
Excel.file |
A .xlsx file derived manually containing single columns of 'signal' from both positive and negative controls. |
effect.size |
A simulated effect size for virtual experiments (e.g., 0.75 is a compound that simulates 75 percents efficacy when compared to the positive control condition). |
bg.ratio |
Numerical minimal 'signal' cutoff value (arbitrary units) applied to isolate fluorescent/luminescent signal above this value. Default value is 3946. |
dic.output |
Output directory.Default is current working directory. |
SampleSize.csv A .csv file of minimum sample sizes required to observe simulated effect sizes based on varied TypeI/TypeII errors.
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