SAMPLE: Sample Size Determination

In mummlab/ARQiv: : ARQiv-HTS,Preparing for and Completing Whole-Organism Screening at High-througput rates

Description

This function calculates the minimum sample size required for your assay with varying paremeters, such as simulated effect size and TypeI/TypeII error rates. This can be completed using .XML or .CSV file formats.

Usage

SAMPLE(Pos.file = NULL, Neg.file = NULL, Roy.file = NULL,
  Excel.file = NULL, effect.size = NULL, bg.ratio = NULL,
  dic.output = NULL)

Arguments

Pos.file	A .xml file derived from your positive control condition, i.e., that with the highest value.
Neg.file	A .xml file derived from your negative control condition, i.e., that with the lowest value.
Roy.file	A .xml file of raw signal values from a 96-well plate of non-transgenic Roy larvae.
Excel.file	A .xlsx file derived manually containing single columns of 'signal' from both positive and negative controls.
effect.size	A simulated effect size for virtual experiments (e.g., 0.75 is a compound that simulates 75 percents efficacy when compared to the positive control condition).
bg.ratio	Numerical minimal 'signal' cutoff value (arbitrary units) applied to isolate fluorescent/luminescent signal above this value. Default value is 3946.
dic.output	Output directory.Default is current working directory.

Value

SampleSize.csv A .csv file of minimum sample sizes required to observe simulated effect sizes based on varied TypeI/TypeII errors.

mummlab/ARQiv documentation built on May 23, 2019, 8:22 a.m.