SSMD.QC: SSMD_QC Calculation

In mummlab/ARQiv: : ARQiv-HTS,Preparing for and Completing Whole-Organism Screening at High-througput rates

Description

This function calculates SSMD_EST of your chosen assay parameters, using both positive and negative control data. This can be completed using .XML or .CSV file formats.

Usage

SSMD.QC(Pos.file = NULL, Roy.file = NULL, bg.ratio = NULL,
  Neg.file = NULL, Excel.file = NULL, dic.output = NULL)

Arguments

Pos.file	A .xml file derived from your positive control condition, i.e., that with the highest value.
Roy.file	A .xml file of raw signal values from a 96-well plate of non-transgenic Roy larvae.
bg.ratio	Numerical minimal 'signal' cutoff value (arbitrary units) applied to isolate fluorescent/luminescent signal above this value.
Neg.file	A .xml file derived from your negative control condition, i.e., that with the lowest value.
Excel.file	A .xlsx file derived manually containing single columns of 'signal' from both positive and negative controls.
dic.output	Output directory.Default is current working directory.
effect.size	A simulated effect size for virtual experiments (e.g., 0.75 is a compound that simulates 75 percents efficacy when compared to the positive control condition).
sampling.num	The number of invidiual wells you will sample from a 96-well plate.
times	The number of iterative cycles of the chosen sampling number.

Value

A .csv file with SSMD_EST estimated via random sampling (i.e., a compound with 75

mummlab/ARQiv documentation built on May 23, 2019, 8:22 a.m.