ddCt: ddCt calculation

In olobiolo/siscreenr: Analyze Genome-Wide siRNA Screening Campaigns

Description

Calculate gene expression levels in a qPCR reaction.

Usage

ddCt(x, ref, types, output = c("ddCt", "relative"), eff = c(2, 2))

Arguments

x	Ct values of measured gene; numeric vector
ref	Ct values of reference gene; numeric vector
types	sample types; character vector or factor
output	type of output, either ddCt or relative template amount (2 ^ -ddCt)
eff	primer efficiencies; numeric vector of length 2

Value

Named numeric vector of either exponents or relative template amounts. Names are taken from types.

olobiolo/siscreenr documentation built on Nov. 26, 2021, 3:08 p.m.