buildAnnotationDatabase: Build a local annotation database for metaseqR2

In pmoulos/metaseqR2-local: An R package for the analysis and result reporting of RNA-Seq data by combining multiple statistical algorithms

Build a local annotation database for metaseqR2

Description

This function creates a local annotation database to be used with metaseqr2 so as to avoid long time on the fly annotation downloads and formatting.

Usage

    buildAnnotationDatabase(organisms, sources,
    db = file.path(system.file(package = "metaseqR2"),
        "annotation.sqlite"),
    forceDownload = TRUE, rc = NULL)

Arguments

organisms	a list of organisms and versions for which to download and build annotations. Check the main metaseqr2 help page for details on supported organisms and the Details section below.
sources	a character vector of public sources from which to download and build annotations. Check the main metaseqr2 help page for details on supported annotation sources.
db	a valid path (accessible at least by the current user) where the annotation database will be set up. It defaults to system.file(package = "metaseqR2"), "annotation.sqlite") that is, the installation path of metaseqR2 package. See also Details.
forceDownload	by default, buildAnnotationDatabase will not download an existing annotation again (FALSE). Set to TRUE if you wish to update the annotation database for a particular version.
rc	fraction (0-1) of cores to use in a multicore system. It defaults to NULL (no parallelization). Sometimes used for building certain annotation types.

Details

Regarding the organisms argument, it is a list with specific format which instructs buildAnnotationDatabase on which organisms and versions to download from the respective sources. Such a list may have the format: organisms=list(hg19=75, mm9=67, mm10=96:97) This is explained as follows:

• A database comprising the human genome versions hg19 and the mouse genome versions mm9, mm10 will be constructed.

• If "ensembl" is in sources, version 75 is downloaded for hg19 and versions 67, 96, 97 for mm9, mm10.

• If "ucsc" or "refseq" are in sources, the latest versions are downloaded and marked by the download date. As UCSC and RefSeq versions are not accessible in the same way as Ensembl, this procedure cannot always be replicated.

organisms can also be a character vector with organism names/versions (e.g. organisms = c("mm10","hg19")), then the latest versions are downloaded in the case of Ensembl.

Regarding db, this controls the location of the installation database. If the default is used, then there is no need to provide the local database path to any function that uses the database (e.g. the main metaseqr2). Otherwise, the user will either have to provide this each time, or the annotation will have to be downloaded and used on-the-fly.

Value

The function does not return anything. Only the SQLite database is created or updated.

Author(s)

Panagiotis Moulos

Examples

# Build a test database with one genome
myDb <- file.path(tempdir(),"testann.sqlite")

organisms <- list(mm10=75)
sources <- "ensembl"

# If the example is not running in a multicore system, rc is ignored
#buildAnnotationDatabase(organisms,sources,db=myDb,rc=0.5)

# A more complete case, don't run as example
# Since we are using Ensembl, we can also ask for a version
#organisms <- list(
#    mm9=67,
#    mm10=96:97,
#    hg19=75,
#    hg38=96:97
#)
#sources <- c("ensembl", "refseq")

## Build on the default location (depending on package location, it may
## require root/sudo)
#buildAnnotationDatabase(organisms,sources)

## Build on an alternative location
#myDb <- file.path(path.expand("~"),"my_ann.sqlite")
#buildAnnotationDatabase(organisms,sources,db=myDb)

pmoulos/metaseqR2-local documentation built on May 21, 2024, 3:46 a.m.