Description Usage Arguments Format Value References
This function reads output from the cuffcompare specific tmap format. It is an tab delimited file that lists the most closely matching reference transcripts for each Cufflinks transcript.
1 | readTmap(filename)
|
filename |
location of the cuffcompare output file in .tmap format |
Tab delimited file, where one row represents a Cufflinks transcripts with the following attributes:
Reference_gene_name
The gene_name attribute of the reference GTF record for this transcript, if present. Otherwise gene_id is used.
Referenc_transcript_id
The transcript_id attribute of the reference GTF record for this transcript
Class_code
The type of relationship between the Cufflinks transcripts and the reference transcript
Cufflinks_gene_id
The Cufflinks internal gene id
Cufflinks_transcript_id
The Cufflinks internal transcript id
FMI
Fraction of major isoform: the expression of this transcript expressed as a fraction of the major isoform for the gene. Ranges from 1 to 100.
FPKM
The expression of this transcript expressed in FPKM
FPKM_conf_lo
The lower limit of the 95% FPKM confidence interval
FPKM_conf_hi
The upper limit of the 95% FPKM confidence interval
coverage
The estimated average depth of read coverage across the transcript.
length
The length of the transcript.
major_isoform_ID
The Cufflinks ID of the gene's major isoform
ref_match_len
The length of the matching positions between the cufflinks and the reference transcript
dataframe |
content of the parsed file as dataframe |
Cufflinks Manual <cuff_in>.tmap: http://cufflinks.cbcb.umd.edu/manual.html#tmap
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