Description Usage Arguments Format Value References
This function reads output from the cuffcompare specific tmap format. It is an tab delimited file that lists the most closely matching reference transcripts for each Cufflinks transcript.
1 | readTmap(filename)
|
filename |
location of the cuffcompare output file in .tmap format |
Tab delimited file, where one row represents a Cufflinks transcripts with the following attributes:
Reference_gene_nameThe gene_name attribute of the reference GTF record for this transcript, if present. Otherwise gene_id is used.
Referenc_transcript_idThe transcript_id attribute of the reference GTF record for this transcript
Class_codeThe type of relationship between the Cufflinks transcripts and the reference transcript
Cufflinks_gene_idThe Cufflinks internal gene id
Cufflinks_transcript_idThe Cufflinks internal transcript id
FMIFraction of major isoform: the expression of this transcript expressed as a fraction of the major isoform for the gene. Ranges from 1 to 100.
FPKMThe expression of this transcript expressed in FPKM
FPKM_conf_loThe lower limit of the 95% FPKM confidence interval
FPKM_conf_hiThe upper limit of the 95% FPKM confidence interval
coverageThe estimated average depth of read coverage across the transcript.
lengthThe length of the transcript.
major_isoform_IDThe Cufflinks ID of the gene's major isoform
ref_match_lenThe length of the matching positions between the cufflinks and the reference transcript
dataframe |
content of the parsed file as dataframe |
Cufflinks Manual <cuff_in>.tmap: http://cufflinks.cbcb.umd.edu/manual.html#tmap
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