readTmap: reads output in .tmap format from cuffcompare

Description Usage Arguments Format Value References

Description

This function reads output from the cuffcompare specific tmap format. It is an tab delimited file that lists the most closely matching reference transcripts for each Cufflinks transcript.

Usage

1
readTmap(filename)

Arguments

filename

location of the cuffcompare output file in .tmap format

Format

Tab delimited file, where one row represents a Cufflinks transcripts with the following attributes:

Reference_gene_name

The gene_name attribute of the reference GTF record for this transcript, if present. Otherwise gene_id is used.

Referenc_transcript_id

The transcript_id attribute of the reference GTF record for this transcript

Class_code

The type of relationship between the Cufflinks transcripts and the reference transcript

Cufflinks_gene_id

The Cufflinks internal gene id

Cufflinks_transcript_id

The Cufflinks internal transcript id

FMI

Fraction of major isoform: the expression of this transcript expressed as a fraction of the major isoform for the gene. Ranges from 1 to 100.

FPKM

The expression of this transcript expressed in FPKM

FPKM_conf_lo

The lower limit of the 95% FPKM confidence interval

FPKM_conf_hi

The upper limit of the 95% FPKM confidence interval

coverage

The estimated average depth of read coverage across the transcript.

length

The length of the transcript.

major_isoform_ID

The Cufflinks ID of the gene's major isoform

ref_match_len

The length of the matching positions between the cufflinks and the reference transcript

Value

dataframe

content of the parsed file as dataframe

References

Cufflinks Manual <cuff_in>.tmap: http://cufflinks.cbcb.umd.edu/manual.html#tmap


psikon/cuffgo documentation built on May 26, 2019, 10:32 a.m.