Description Usage Arguments Format References Examples
This wrapper function creates from a dataframe processed in R an GTF File in cufflinks specific GTF format. There will be different fields in GTF Files for every program of the cufflinks suite, so you have to choose the right program to save your data.
1 2 | saveToGTF(filename, program = "cufflinks", dataframe)
|
filename |
path to the save location |
program |
for which program the GTF File will be created (default="cufflinks") |
dataframe |
saves this dataframe |
All three programs have 8 standard tab delimited values that have to be provided by every programm and a variable attribute field that is separated by semicolons. this attribute field is different between all three programs. Standard fields:
chr
Chromosome or contig name
program
The name of the program that generated this file
feature
The type of record (always either "transcript" or "exon"
start
The leftmost coordinate of this record
end
The rightmost coordinate of this record
score
The most abundant isoform for each gene is assigned a score of 1000. Minor isoforms are scored by the ratio (minor FPKM/major FPKM)
strand
Cufflinks' guess for which strand the isoform came from. Always one of "+", "-", "."
frame
deprecated - field not used
cufflinks specific attributes:
gene_id
Cufflinks gene id
transcript_id
Cufflinks transcript id
exon_number
number of exon for this transcript
fpkm
Isoform-level relative abundance in Fragments Per Kilobase of exon model per Million mapped fragment
frac
deprecated value
conf_lo
Lower bound of the 95% confidence interval of the abundance of this isoform, as a fraction of the isoform abundance. That is, lower bound = FPKM * (1.0 - conf_lo)
conf_hi
Upper bound of the 95% confidence interval of the abundance of this isoform, as a fraction of the isoform abundance. That is, upper bound = FPKM * (1.0 + conf_lo)
cov
Estimate for the absolute depth of read coverage across the whole transcript
full_read_support
When RABT assembly is used, this attribute reports whether or not all introns and internal exons were fully covered by reads from the data.
cuffcompare specific attributes:
gene_id
Cufflinks gene id
transcript_id
Cufflinks transcript id
exon_number
number of exon for this transcript
gene_name
The gene_name attribute of the reference GTF record for this transcript, if present. Otherwise gene_id is used.
oId
old identifier of this gene e.g. in cufflinks output
nearest_ref
The reference transcript to which the class code refers, if any
class_code
The type of relationship between the Cufflinks transcripts and the reference transcript
tss_id
The tss_id associated with the object, or "-" if not a transcript/primary transcript, or if tss_id isn't present
cuffmerge specific attributes:
gene_id
Cufflinks gene id
transcript_id
Cufflinks transcript id
exon_number
number of exon for this transcript
gene_name
The gene_name attribute of the reference GTF record for this transcript, if present. Otherwise gene_id is used.
oId
old identifier of this gene e.g. in cufflinks output
contained_in
is this transcript contained in a nother bigger transcript
nearest_ref
The reference transcript to which the class code refers, if any
class_code
The type of relationship between the Cufflinks transcripts and the reference transcript
tss_id
The tss_id associated with the object, or "-" if not a transcript/primary transcript, or if tss_id isn't present
CUfflinks Manual: http://cufflinks.cbcb.umd.edu/manual.html
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