Description Usage Arguments Details Value Author(s) See Also Examples
Given a linear model fit to microarray data, compute estimated coefficients and standard errors for a given set of contrasts.
1 | contrasts.fit(fit, contrasts=NULL, coefficients=NULL)
|
fit |
an |
contrasts |
numeric matrix with rows corresponding to coefficients in |
coefficients |
vector indicating which coefficients are to be kept in the revised fit object. An alternative way to specify the |
This function accepts input from any of the functions lmFit
, lm.series
, mrlm
, gls.series
or lmscFit
.
The function re-orientates the fitted model object from the coefficients of the original design matrix to any set of contrasts of the original coefficients.
The coefficients, unscaled standard deviations and correlation matrix are re-calculated in terms of the contrasts.
The idea of this function is to fit a full-rank model using lmFit
or equivalent, then use contrasts.fit
to obtain coefficients and standard errors for any number of contrasts of the coefficients of the original model.
Unlike the design matrix input to lmFit
, which normally has one column for each treatment in the experiment, the matrix contrasts
may have any number of columns and these are not required to be linearly independent.
Methods of assessing differential expression, such as eBayes
or classifyTestsF
, can then be applied to fitted model object.
The coefficients
argument provides a simpler way to specify the contrasts
matrix when the desired contrasts are just a subset of the original coefficients.
Warning. For efficiency reasons, this function does not re-factorize the design matrix for each probe. A consequence is that, if the design matrix is non-orthogonal and the original fit included quality weights or missing values, then the unscaled standard deviations produced by this function are approximate rather than exact. The approximation is usually acceptable. The results are always exact if the original fit was a oneway model.
An list object of the same class as fit
, usually MArrayLM
. This is a list with components
coefficients |
numeric matrix containing the estimated coefficients for each contrast for each probe. |
stdev.unscaled |
numeric matrix conformal with |
|
numeric |
... |
any other components found in |
Gordon Smyth
An overview of linear model functions in limma is given by 06.LinearModels.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 | # Simulate gene expression data: 6 microarrays and 100 genes
# with one gene differentially expressed in first 3 arrays
M <- matrix(rnorm(100*6,sd=0.3),100,6)
M[1,1:3] <- M[1,1:3] + 2
# Design matrix corresponds to oneway layout, columns are orthogonal
design <- cbind(First3Arrays=c(1,1,1,0,0,0),Last3Arrays=c(0,0,0,1,1,1))
fit <- lmFit(M,design=design)
# Would like to consider original two estimates plus difference between first 3 and last 3 arrays
contrast.matrix <- cbind(First3=c(1,0),Last3=c(0,1),"Last3-First3"=c(-1,1))
fit2 <- contrasts.fit(fit,contrast.matrix)
fit2 <- eBayes(fit2)
# Large values of eb$t indicate differential expression
results <- classifyTestsF(fit2)
vennCounts(results)
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