View source: R/scraps_to_seurat.R
scraps_to_matrix | R Documentation |
Read scraps output from umi_tools to sparseMatrix
scraps_to_matrix( file, n_min = 5, gene_min = 5, alt_only = FALSE, filter_low = TRUE, cell_ids = NULL, types = "3'UTR", types2 = NULL, pf = NULL )
file |
scraps output table containing |
n_min |
minimum number of observations for a site to be kept |
gene_min |
minimum number of observations for gene to be kept |
alt_only |
if TRUE, only keep genes with alternative polyA sites |
filter_low |
if TRUE, remove low genes and cell ids, if FALSE, set to -1 |
cell_ids |
if given, use only these cell barcodes, and fill in empty ones |
types |
filter to only these types of PA sites, set to NULL to use all |
types2 |
another filter to only these types of PA sites, set to NULL to use all |
pf |
data.frame with SAF first column and position factor by gene, calculated by |
count matrix
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