ProjectIntegration | R Documentation |
The main steps of this procedure are outlined below. For a more detailed description of the methodology, please see Hao, et al Biorxiv 2022: \Sexpr[results=rd]{tools:::Rd_expr_doi("10.1101/2022.02.24.481684")}
ProjectIntegration(
object,
sketched.assay = "sketch",
assay = "RNA",
reduction = "integrated_dr",
features = NULL,
layers = "data",
reduction.name = NULL,
reduction.key = NULL,
method = c("sketch", "data"),
ratio = 0.8,
sketched.layers = NULL,
seed = 123,
verbose = TRUE
)
object |
A Seurat object with all cells for one dataset |
sketched.assay |
Assay name for sketched-cell expression (default is 'sketch') |
assay |
Assay name for original expression (default is 'RNA') |
reduction |
Dimensional reduction name for batch-corrected embeddings in the sketched object (default is 'integrated_dr') |
features |
Features used for atomic sketch integration |
layers |
Names of layers for correction. |
reduction.name |
Name to save new reduction as; defaults to
|
reduction.key |
Key for new dimensional reduction; defaults to creating
one from |
method |
Methods to construct sketch-cell representation for all cells (default is 'sketch'). Can be one of:
|
ratio |
Sketch ratio of data slot when |
sketched.layers |
Names of sketched layers, defaults to all
layers of “ |
seed |
A positive integer. The seed for the random number generator, defaults to 123. |
verbose |
Print progress and message |
First learn a atom dictionary representation to reconstruct each cell. Then, using this dictionary representation, reconstruct the embeddings of each cell from the integrated atoms.
Returns a Seurat object with an integrated dimensional reduction
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