Description Usage Arguments Value
This function will take a cds and add sample levels phenodata to all the cells from that
1 | add_meta_data_cds(cds, meta, cds_col = "sample", meta_col = "sample")
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folders |
A vector of cellranger folders (full folder name is ideal). These must contain an "outs" subfolders with two files; 1) raw_feature_bc_matrix.h5, and 2) filtered_feature_bc_matrix.h5. For aggregated samples run with cellrangers 'aggregate' function, this folder should also contain the aggregation.csv file. |
samplenames |
An optional vector that corresponds to the names you would like to give to each element in filelist. This defaults to the the basename of the filelist element i.e. |
unfiltered |
This parameter, if true, returns a list containing 1) the filtered cds, 2) a list of unfiltered cds for each sample in 'folders'. |
empty.droplet.threshold |
minimum number of umis per droplet to include in unfiltered output (default 15) |
expressed_genes |
If true, this option removes genes that do not have expression in at least a minumum number of cells (cell_min parameter) |
cell_min |
Minimum number of cells that a gene needs to be expressed for the gene to be included in the |
aggregated |
will read a cellranger aggregate folder, this is currently only supported for 1 folder. The 'aggregation.csv' file must be included in the 'outs' folder. |
a cell_data_set object or a list of items if unfiltered data is returned (see unfiltered)
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