This dataset contains the analysis data for each of the 92 synthetic RNAs included in the ERCC ExFold spike-in mix (the same RNAs are used for Mix 1 and Mix 2). This spike-in mix is the most commonly used way to included spike-in control sequences into an RNA-Seq experiment. The catalog number at ThermoFisher is 4456739. Each mix has four groups of RNAs with defined ratios between Mix 1 vs Mix 2: Group A is 4:1; Group B is 1:1; Group C is 2:3; and Group D is 1:2. Each RNA within each group has a defined concentration per microliter. The total molar concentration for Mixes 1 and 2 are the same (approximately 100 nM), though the total mass concentrations are slightly different.
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A data frame with 92 rows and 9 columns:
the name of the RNA (same as the sequence names in
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