Description Usage Arguments Details Value Author(s) Examples
After loading CyTOF data as FCS, updates the parameters slot of the flowFrame so that isotopes & channel name are clearly separated; adds a type column to explicitly set parameter type
1 | make.cytoFrame(ff, channels)
|
ff |
a flowFrame object |
channels |
a list of channels sorted by type (see details) |
Channels
a flowFrame object with extra type and isotope columns
Yann Abraham
1 2 3 4 5 6 7 8 9 10 | ff <- read.FCS(system.file("extdata","Staurosporine_cd4+_H05.fcs",package="cytoCore"))
channels <- list(CyTOF=c('Time','Cell_length'),
DNA=c('DNA-1','DNA-2'),
phenotypic=c('CD3','CD45','CD4','CD20','CD33','CD123','CD14','HLA-DR','IgM','CD7'),
functional=c('pNFkB','pp38','pStat5','pAkt','pStat1','pSHP2','pZap70','pStat3','pSlp76','pBtk','pPlcg2','pErk','pLat','pS6'),
barcode=c('BC1','BC2','BC3','BC4','BC5','BC6','BC7')
)
cf <- make.cytoFrame(ff,channels)
|
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