ExonTesting: "ExonTesting"

In REIDS: Random Effects for the Identification of Differential Splicing

Description

The ExonTesting function performs a t-test on the array score of predefined groups. If specified, probesets are filtered out on exon scores and test significance.

Usage

ExonTesting(Data, Exonthreshold = NULL, groups = list(group1 = NULL, group2
  = NULL), paired = FALSE, significancelevel = NULL)

Arguments

Data	The Data on which testing of the array scores should be conducted. This is preferably output of the REIDS function
Exonthreshold	The exon score threshold to be maintained. If not NULL, probesets with an exon score lower than this value are not considered further and the p-values will be adjusted for multiplicity after testing. If NULL, all probesets are considered and a multiplicity correction is not performed.
groups	A list with two elements speficifing the columns of the data of group 1 in group1 and those of group 2 in group2.
paired	Logical. Are the groups paired? If TRUE the mean paired differences are calculated and tested whether these are significantly different from zero or not.
significancelevel	The significance level to be maintained on the p-values. The filtering on the significance is conducted only if an Exonthreshold is specified and the p-value are adjusted for multiplicity.

Value

A data frame with one line per exon. The columns contain the gene ID, the exon ID, the test statistic, a p-value and an adjusted p-value. If the groups are paired also the mean paired difference is given. The p-values are adjusted for multiplicity and filtered on significance if significancelevel is not NULL.

REIDS documentation built on May 2, 2019, 4:45 p.m.