RG: Experiment Data: RGList\_CALIB Example

In CALIB: Calibration model for estimating absolute expression levels from microarray data

Description

This RGList_CALIB object represents microarray data of two arrays.

It is generated by function read.rg from raw data files.

Usage

data(RG)

Format

RG is an RGList_CALIB object containing the following list components: \$R,\$G,\$Rb,\$Gb,
\$RArea,\$GArea,\$targets,\$source and \$genes. It represents two microarrays and 19749 clones.

Source

This comes from a publicly avaible dataset, consisting 14 hybridizations. From this RG, two out of these fourteen are chosen. The experiment design of these two are color flip. Except for cDNA probes, external control spikes are also spotted on the arrays. There are 192 ratio controls, 480 calibration controls,24 negative controls and 72 utility controls.

For more information, see the reference.

References

Engelen, K., Naudts, B., DeMoor, B., Marchal, K. (2006) A calibration method for estimating absolute expression levels from microarray data. Bioinformatics 22: 1251-1258.

Hilson,P.,et al. (2004) Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications. Genome Res. 14, 2176-2189.

Examples

data(RG)

CALIB documentation built on Oct. 31, 2019, 3:45 a.m.